Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Poor postoperative pain control is associated with prolonged length of stay after elective spine surgery: An observational cohort study.

North American Spine Society journal·2026
Same author

Increased Ih Current Is Associated with Reduced Hippocampal CA1 Excitability in a Mouse Model of Multiple Sclerosis.

eNeuro·2026
Same author

Pathological disruption of CELF2 shuttling causes neuronal hyperactivity, learning deficits, and seizures.

The Journal of clinical investigation·2026
Same author

Remembering Leo P. Renaud: Mentor, physiologist, friend.

Journal of neuroendocrinology·2026
Same author

A Case of Vertical Diplopia after Thalamic Deep Brain Stimulation for Essential Tremor.

Movement disorders clinical practice·2025
Same author

Modulation of NMDA receptor signaling and zinc chelation prevent seizure-like events in a zebrafish model of SLC13A5 epilepsy.

PLoS biology·2025

Related Experiment Video

Updated: Dec 22, 2025

Derivation of a Human Brain Organoid with Microglia Development
10:34

Derivation of a Human Brain Organoid with Microglia Development

Published on: January 17, 2025

1.8K

Embryonic microglia influence developing hypothalamic glial populations.

Candace M Marsters1,2,3,4, Dinushan Nesan1,3,4, Rena Far1,3,4

  • 1Department of Medical Genetics, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.

Journal of Neuroinflammation
|May 8, 2020
PubMed
Summary
This summary is machine-generated.

Embryonic microglia regulate glial cell development in the hypothalamus. Removing microglia delays glial progenitor cell migration and alters oligodendrocyte progenitor cell behavior, impacting neurodevelopment.

Keywords:
AstrocytesChemokinesCytokinesHypothalamusMicrogliaNeural developmentNeural progenitor cellsOligodendrocyte progenitor cellsOligodendrocytes

More Related Videos

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
09:12

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

16.6K
Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders
05:00

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders

Published on: November 11, 2022

2.8K

Related Experiment Videos

Last Updated: Dec 22, 2025

Derivation of a Human Brain Organoid with Microglia Development
10:34

Derivation of a Human Brain Organoid with Microglia Development

Published on: January 17, 2025

1.8K
Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
09:12

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

16.6K
Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders
05:00

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders

Published on: November 11, 2022

2.8K

Area of Science:

  • Neuroscience
  • Immunology
  • Developmental Biology

Background:

  • Historically, fetal microglia were considered immature.
  • Emerging evidence shows purposeful interactions between fetal microglia and neural progenitors.
  • The tuberal hypothalamus is crucial for energy balance, reproduction, and thermoregulation.

Purpose of the Study:

  • To investigate the influence of embryonic microglia on gliogenesis in the developing tuberal hypothalamus.
  • To understand the role of microglia in the differentiation and migration of glial cells.

Main Methods:

  • Immunohistochemistry to quantify glial cells (E13.5-E17.5).
  • Pharmacological knockdown of fetal microglia using PLX5622.
  • Cytokine/chemokine analysis and neurosphere assays to assess progenitor cell behavior.

Main Results:

  • Activated microglia were found near Olig2+ neural progenitor cells (NPCs) in the embryonic hypothalamus.
  • Microglia absence led to increased Olig2+ cells at the ventricle and decreased mantle zone cells, indicating delayed migration.
  • Microglia absence reduced specific cytokine/chemokine levels (e.g., CCL2, CXCL10), which in turn increased NPC proliferation and differentiation.

Conclusions:

  • Embryonic microglia play a significant role in regulating gliogenesis within the developing tuberal hypothalamus.
  • Microglia influence glial progenitor cell migration and differentiation through cytokine signaling.