Catellatospora sichuanensis sp. nov., a novel actinobacterium isolated from soil

A novel actinobacterium, designated strain H14505^T, was isolated from a soil sample collected in Hong Yuan, Sichuan, southwest PR China. The temperature, pH and NaCl ranges for growth were determined to be 15-35 °C (optimum, 28 °C), 6.0-8.0 (optimum, pH 7.0) and 0-2 % (w/v; optimum without NaCl), respectively. The polar lipdis detected for strain H14505^T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, glycolipid and four unidentified lipids. The predominant menaquinones of strain H14505^T were MK-9(H₄) and MK-9(H₆), and the prevalent fatty acids (>10 %) were C_18 : 1 ω9c, C_17 : 1 ω8c, summed feature 5 (anteiso-C_18 : 0/ C_18 : 2 ω6,9c) and C_16 : 0. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences indicated that strain H14505^T showed high similarity to Catellatospora vulcania NEAU-JM1^T (99.0 %) and Catellatospora paridis NEAU-CL2^T (99.0 %), and formed a monophyletic clade within the the genus Catellatospora in the phylogenetic trees. However, the average nucleotide indentity and DNA-DNA hybridization values between strain H14505^T and closely related Catellatospora species showed that it belonged to a distinct species. Furthermore, the results of morphological, physiological and biochemical tests allowed further phenotypic differentiation of strain H14505^T from its closest relatives. Thus, it is proposed that strain H14505^T represents a novel species of the genus Catellatospora, for which the name Catellatospora sichuanensis sp. nov. is proposed. The type strain of Catellatospora sichuanensis is H14505^T (=JCM 32394^T=CICC 11042^T).