Oleiharenicola lentus sp. nov., isolated from irrigation water

Bacterial strain TWA-58^T, isolated from irrigation water in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain TWA-58^T formed a phylogenetic lineage in the genus Oleiharenicola of the family Opitutaceae. Strain TWA-58^T was most closely related to Oleiharenicola alkalitolerans NVT^T with a 96.7 % 16S rRNA gene sequence similarity. Strain TWA-58^T showed 75.2 % average nucleotide identity, 70.9 % average amino acid identity and 21.0 % digital DNA-DNA hybridization identity with O. alkalitolerans NVT^T. Cells were Gram-stain-negative, aerobic, motile, coccoid-shaped and formed transparent colonies. Optimal growth occurred at 25 °C, pH 6, and 0 % NaCl. The major fatty acids of strain TWA-58^T were iso-C_15 : 0 and anteiso-C_15 : 0. The predominant hydroxy fatty acid was iso-C_13 : 0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminophospholipids. The major isoprenoid quinone was MK-7. Genomic DNA G+C content of strain TWA-58^T was 65.3 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TWA-58^T should be classified in a novel species of the genus Oleiharenicola, for which the name Oleiharenicola lentus sp. nov. is proposed. The type strain is TWA-58^T (=BCRC 81161^T=LMG 31019^T=KCTC 62872^T).