Two quick methods for isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase

Methods are described which allow the isolation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (rubisco) in a very short time. Source of the material was highly impure commercial enzyme in the case of spinach rubisco or bacteria grown from a fermentor in the case of Alcaligenes eutrophus rubisco. Purity of the enzymes is demonstrated by gel electrophoreses. Enzyme isolated from fresh cells gave crystals of excellent diffraction, suitable for X-ray structure analyisis.