Related Experiment Video
Updated: Dec 22, 2025
A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
Published on: July 3, 2013
Construction of ceRNA Coexpression Network and Screening of Molecular Targets in Colorectal Cancer
Zhao Hui1, Wang Zhanwei2, Yang Xi3
1Department of Gastroenterology, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, 198 Hongqi Road, Huzhou, Zhejiang, China 313000.
Objective:
To screen some RNAs that correlated with colorectal cancer (CRC).
Methods:
Differentially expressed miRNAs, lncRNAs, and mRNAs between cancer tissues and normal tissues in CRC were identified using data from the Gene Expression Omnibus (GEO) database. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and protein-protein interactions (PPIs) were performed to do the functional enrichment analysis. And a lncRNA-miRNA-mRNA network was constructed which correlated with CRC. RNAs in this network were subjected to analyze the relationship with the patient prognosis.
Results:
A total of 688, 241, and 103 differentially expressed genes (diff-mRNA), diff-lncRNA, and diff-miRNA were obtained between cancer tissues and normal tissues. A total of 315 edges were obtained in the ceRNA network. lncRNA RP11-108K3.2 and mRNA ONECUT2 correlated with prognosis.
Conclusion:
The identified RNAs and constructed ceRNA network could provide great sources for the researches of therapy of the CRC. And the lncRNA RP11-108K3.2 and mRNA ONECUT2 may serve as a novel prognostic predictor of CRC.
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