Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impulsive choices in mice lacking imprinted Nesp55.

Genes, brain, and behavior·2016
Same author

Imprinting the Gnas locus.

Cytogenetic and genome research·2006
Same author

A new module arrangement for plasmapheresis.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2002
Same author

The contactin-related protein FAR-2 defines purkinje cell clusters and labels subpopulations of climbing fibers in the developing cerebellum.

Molecular and cellular neurosciences·2001
Same author

Neurotractin, a novel neurite outgrowth-promoting Ig-like protein that interacts with CEPU-1 and LAMP.

The Journal of cell biology·1999
Same author

The gene of the neural cell recognition molecule F11: conserved exon-intron arrangement in genes of neural members of the immunoglobulin superfamily.

Gene·1997

Related Experiment Video

Updated: Dec 22, 2025

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

7.5K

Assessing Human Embryonic Stem Cell-Derived Dopaminergic Neuron Progenitor Transplants Using Non-invasive Imaging

M Mousavinejad1, S Skidmore1,2, F G Barone1

  • 1Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, L69 3BX, UK.

Molecular Imaging and Biology
|May 8, 2020
PubMed
Summary
This summary is machine-generated.

Human pluripotent stem cell-derived dopaminergic progenitor cells show promise for Parkinson's disease therapy. Imaging confirmed transplanted cells remained localized and differentiated, indicating a safe treatment approach.

Keywords:
BioluminescenceDopaminergic neuron progenitor cellsHuman pluripotent stem cellMagnetic resonance imagingNon-invasive imagingParkinson’s disease

More Related Videos

Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons
09:21

Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons

Published on: July 7, 2023

1.9K
Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain
06:12

Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain

Published on: January 27, 2022

3.5K

Related Experiment Videos

Last Updated: Dec 22, 2025

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

7.5K
Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons
09:21

Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons

Published on: July 7, 2023

1.9K
Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain
06:12

Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain

Published on: January 27, 2022

3.5K

Area of Science:

  • Neuroscience
  • Regenerative Medicine
  • Biomedical Imaging

Background:

  • Human pluripotent stem cell-derived dopaminergic progenitor cells (hPSC-DAPCs) offer a potential Parkinson's disease (PD) therapy.
  • Intracranial transplantation of these cells raises safety concerns regarding proliferation, migration, and inflammation.

Purpose of the Study:

  • To investigate the in vivo fate and safety of hPSC-DAPC transplants in the rat striatum using a bimodal imaging approach.
  • To assess the long-term engraftment and differentiation of transplanted cells.

Main Methods:

  • hPSC-DAPCs were labeled with luciferase/ZsGreen or MPIOs and transplanted into the rat striatum.
  • In vivo tracking was performed using bioluminescence imaging and magnetic resonance (MR) imaging.
  • Post-mortem histological analysis was conducted to evaluate cell differentiation and glial reactivity.

Main Results:

  • Bioluminescence signal attenuated by week 2, but MR imaging showed MPIO-labeled DAPCs remained at the injection site for over 120 days.
  • Histological analysis confirmed that cell labeling did not impede differentiation into mature dopaminergic neurons.
  • No increased glial reactivity was observed in labeled cells compared to controls.

Conclusions:

  • The study supports the safety of transplanting hPSC-derived DAPCs for Parkinson's disease treatment.
  • Bimodal imaging effectively tracked cell survival and integration.
  • Labeled cells demonstrated appropriate differentiation and integration without adverse inflammatory responses.