Enterocin M-Producing Enterococcus faecium CCM 8558 Demonstrating Probiotic Properties in Horses

Area of Science:

• Veterinary Microbiology
• Animal Nutrition
• Gut Microbiome Research

Background:

• Enterococcus faecium AL41 (CCM 8558) is a probiotic strain known for benefits in various animals.
• Its effects on equine health, specifically gut microbiota and immune function, require detailed investigation.

Purpose of the Study:

• To evaluate the impact of Enterococcus faecium CCM 8558 on the digestive tract microbiota, immune parameters, and biochemical markers in horses.
• To confirm the colonization and efficacy of this probiotic strain in a equine model.

Main Methods:

• Administration of Enterococcus faecium CCM 8558 to horses over a 14-day period.
• Monitoring of E. faecium CCM 8558 counts via PCR and CFU/g analysis.
• Assessing phagocytic activity (PA), hydrolytic enzyme activities (cellulolytic, xylanolytic, pectinolytic, amylolytic, inulolytic), and dry matter content in fecal samples.

Main Results:

• E. faecium CCM 8558 successfully colonized the equine digestive tract, reaching significant counts.
• A notable reduction in Gram-negative aeromonads and a tendency towards increased phagocytic activity were observed.
• Significant increases in cellulolytic, xylanolytic, pectinolytic, and amylolytic enzyme activities were recorded, indicating improved fiber and carbohydrate digestion.

Conclusions:

• Enterococcus faecium CCM 8558 demonstrates probiotic potential in horses, positively influencing gut microbiota and immune response.
• The strain effectively reduces pathogenic bacteria and enhances digestive capabilities, supporting overall gut health in equines.
• Further studies are warranted to explore additional benefits and optimal application strategies for E. faecium CCM 8558 in horses.