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A chronic jugular catheterization for remote blood sampling in freely moving mink
C Bonnefond1, L G Fouche, L Martinet
1Département de Physiologie Animale, Institut National de la Recherche Agronomique, Jouy en Josas, France.
Physiology & Behavior
|January 1, 1988
Summary
This study details a chronic cannulation technique in minks for blood sampling. The method allows for studying plasma prolactin (PRL) and luteinizing hormone (LH) concentrations under different conditions.
Area of Science:
- Veterinary Science
- Animal Physiology
- Surgical Techniques
Background:
- Chronic catheterization is essential for longitudinal physiological studies in animals.
- Previous methods may have limitations in allowing natural animal movement and preventing catheter damage.
- Understanding hormonal responses to handling and anesthesia is crucial for animal welfare and research integrity.
Purpose of the Study:
- To describe a novel chronic cannulation method for minks.
- To evaluate the impact of handling and anesthesia on plasma prolactin (PRL) and luteinizing hormone (LH) levels in minks using this technique.
Main Methods:
- A silastic catheter was implanted in the jugular vein of minks.
- The catheter was tunneled subcutaneously and emerged dorsally, protected by a spring and external support.
- Swivels were incorporated to prevent catheter twisting during animal movement.
Main Results:
- The described cannulation system facilitated chronic blood sampling in minks.
- The study successfully monitored plasma PRL and LH concentrations.
- Data on hormonal changes related to handling and anesthesia were obtained (specific results not detailed in the abstract).
Conclusions:
- The developed chronic cannulation technique is effective for physiological studies in minks.
- This method allows for the investigation of hormonal dynamics in response to experimental manipulations.
- The system supports animal welfare by allowing natural movement and minimizing catheter-related stress.