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Updated: Dec 22, 2025

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
Published on: May 6, 2022
Epigenome-wide association study reveals CpG sites related to COG of neuroblastoma
Hao Zhao1,2, Xiaojun Zhou3, Hu Sun2
1Department of Neurosurgery, Qilu Hospital of Shandong University and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan 250012, Shandong, China.
Background:
Neuroblastoma (NB) is the most common extracranial solid tumor in infants and children. Its variable location and complex pathogenesis make NB hard for early diagnosis and risk classification.
Methodology:
We analyzed the methylation data of 236 samples from patients with NB in Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database. Kaplan-Meier survival analysis was used for comparing overall survival of NB patients in different groups. Epigenome-wide association study (EWAS) was conducted to screen CpGs significantly associated with NB patients' Children's Oncology Group (COG). Logistic regression method was used for constructing a model to predict NB patients' COG.
Results:
NB patients in low COG showed significantly superior prognosis than those in high COG. A total of seven CpG sites were found closely related to COG. Logistic regression model based on those CpGs showed superior performance in separating NB patients in different COGs.
Conclusions:
The present study highlights the important role of DNA methylation in NB development, which might provide evidence for treatment decisions for children NB.
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