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Long noncoding RNA ANRIL promotes the malignant progression of cholangiocarcinoma by epigenetically repressing ERRFI1

Yang Yu1, Qiaoyu Chen2, Xunlei Zhang1

  • 1Department of Oncology, Affiliated Tumor Hospital of Nantong University, Nantong, China.

Cancer Science
|May 8, 2020
PubMed

Insights

The long noncoding RNA ANRIL promotes cholangiocarcinoma (CCA) by epigenetically silencing the tumor suppressor ERRFI1. Inhibiting ANRIL suppressed CCA cell growth and migration, suggesting ANRIL as a therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Epigenetics

Background:

  • Long noncoding RNAs (lncRNAs) play crucial roles in human cancers.
  • The lncRNA ANRIL's function in cholangiocarcinoma (CCA) is currently unknown.

Purpose of the Study:

  • To investigate the role of ANRIL in cholangiocarcinoma (CCA) development.
  • To explore ANRIL's potential as a therapeutic target for CCA.

Main Methods:

  • RNA sequencing analysis was performed after ANRIL knockdown.
  • The interaction between ANRIL, EZH2, and the ERRFI1 promoter was investigated.
  • Cell proliferation and migration assays were conducted in vitro and in vivo.

Main Results:

  • ANRIL knockdown significantly inhibited CCA cell proliferation and migration.
  • ANRIL epigenetically suppresses the tumor suppressor gene ERRFI1 by interacting with EZH2 and maintaining H3K27me3 levels.
  • RNA sequencing revealed ANRIL knockdown altered genes involved in cell cycle, proliferation, and apoptosis.

Conclusions:

  • ANRIL acts as an oncogenic molecule in CCA through epigenetic regulation.
  • ANRIL is a potential therapeutic target for cholangiocarcinoma intervention.
Keywords:
ANRILERRFI1cholangiocarcinomaepigenetic regulationlong noncoding RNA

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