Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Design Strategies for Developing Chiral Macrocyclic Chelators as a Diagnostic Tool.

Accounts of chemical research·2026

Same author

Influences of Ring Sizes and Chiral Substituents on Macrocyclic Chelators for Improving <sup>68</sup>Ga Radiolabeling.

JACS Au·2026

Same author

Medium matters: modeling the luminescence spectra and emission decay of LaPO<sub>4</sub>:Eu<sup>3+</sup> nanoparticles.

Nanoscale·2025

Same author

Dye-sensitized upconversion nanoparticles with enhanced aqueous luminescence for neuronal imaging.

Nanoscale·2025

Same author

One-Step Assembly of α-Aryl-Substituted DOTAs as Superior and Universal Platforms for Multifunctional Theranostics.

Angewandte Chemie (International ed. in English)·2025

Same author

Cyanine-scaffold fluorogenic probes for visual detection of nitroreductase in living bacteria.

Journal of materials chemistry. B·2025

Search research articles

Related Experiment Video

Updated: Dec 22, 2025

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

Fluorescence Analysis: Shedding Light on Biological Systems.

Ka-Leung Wong¹, Chi-Sing Lee¹, Ga-Lai Law²

¹Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, P. R. China.

|May 8, 2020

Summary

This collection highlights new fluorescent biomolecules and building blocks for advanced applications. It showcases novel probes, nucleoside analogues, and nanocelluloses for enhanced detection and imaging.

More Related Videos

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

Related Experiment Videos

Last Updated: Dec 22, 2025

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

Area of Science:

Organic Chemistry
Materials Science
Biochemistry

Background:

This special collection focuses on fluorescent biomolecules and their building blocks.
It features contributions on novel fluorescent reporters and their applications.

Discussion:

The collection includes lanthanide- and transition-metal-based probes.
It also covers fluorescent nucleoside analogues and fluorescent nanocelluloses.

Key Insights:

Explores the development of new fluorescent reporters.
Highlights diverse applications of fluorescent materials in life sciences.
Showcases cutting-edge research in molecular imaging and sensing.

Outlook:

Future directions in fluorescent probe design.
Potential applications of fluorescent biomolecules in diagnostics and therapeutics.
Advancements in sustainable fluorescent materials like nanocelluloses.