Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A method to estimate binding constants at variable protein concentrations.

J Romer, M H Bickel

The Journal of Pharmacy and Pharmacology

|January 1, 1979

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Barriers and new opportunities in developing effective therapies for diabetic neuropathy: International expert consensus recommendations.

Diabetes research and clinical practice·2025

Same author

[Not Available].

Annales de genetique et de selection animale·2012

Same author

[Not Available].

Annales de genetique et de selection animale·2012

Same author

Immunolymphoscintigraphy for metastatic sentinel nodes: test of a model.

International journal of molecular imaging·2011

Same author

Synergistic effect of the human GLP-1 analogue liraglutide and a dual PPARalpha/gamma agonist on glycaemic control in Zucker diabetic fatty rats.

Diabetes, obesity & metabolism·2009

Same author

Interleukin-20 plays a critical role in maintenance and development of psoriasis in the human xenograft transplantation model.

The British journal of dermatology·2008

Same journal

Uterine relaxant effects of Ageratum conyzoides essential oil: influence of hormonal status and calcium modulation.

The Journal of pharmacy and pharmacology·2026

Same journal

Liraglutide-mediated protection against ethanol-induced gastric ulcer in male and female rats: enhancement of mucosal defense, anti-inflammatory and antioxidant mechanism.

The Journal of pharmacy and pharmacology·2026

Same journal

Exploring the mechanism of action of Qufengzhitong pills in treating rheumatoid arthritis via the PI3K/AKT, NF-κB, and MAPK pathways.

The Journal of pharmacy and pharmacology·2026

Same journal

Mechanistic evidence for PI3K/Akt-driven reactivation of Nrf2/HO-1 by dapagliflozin in acute cisplatin hepatotoxicity.

The Journal of pharmacy and pharmacology·2026

Same journal

Advances in nano-drug delivery systems for targeting therapy of breast cancer.

The Journal of pharmacy and pharmacology·2026

Same journal

Zi Shen decoction inhibits non-small cell lung cancer progression by regulating macrophage polarization via the gut microbiota.

The Journal of pharmacy and pharmacology·2026

See all related articles

A novel method accurately measures drug-protein binding constants for chlorpromazine and imipramine to serum albumin. This technique enhances the study of drug interactions and binding site characterization.

Area of Science:

Biochemistry
Pharmacology
Analytical Chemistry

Background:

Drug-protein binding is crucial for drug efficacy and safety.
Serum albumin is a primary drug-binding protein.
Accurate determination of binding constants is essential for pharmacokinetic studies.

Purpose of the Study:

To introduce a new experimental approach for determining drug-protein association constants.
To accurately measure the binding of chlorpromazine and imipramine to serum albumin.
To validate the method for systems with one or two classes of binding sites.

Main Methods:

A novel experimental approach varying protein concentration instead of ligand concentration.
Determination of association constants for chlorpromazine and imipramine binding to serum albumin at low protein saturation.

Related Experiment Videos

Application of the method to systems with single and multiple binding sites.

Main Results:

The new method accurately determined association constants for chlorpromazine and imipramine with serum albumin.
The approach is effective for systems with one class of binding sites.
The method shows potential for improving accuracy in studies with two classes of binding sites.

Conclusions:

The developed experimental approach offers a reliable method for quantifying drug-protein interactions.
This technique provides a valuable tool for pharmaceutical research and drug development.
The method enhances the accuracy of binding constant determination in complex biological systems.