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Related Concept Videos

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

1.7K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.7K
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

2.8K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
2.8K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.4K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.4K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.6K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.6K
Spin–Spin Coupling Constant: Overview01:08

Spin–Spin Coupling Constant: Overview

1.4K
In bromoethane, the three methyl protons are coupled to the two methylene protons that are three bonds away. In accordance with the n+1 rule, the signal from the methyl protons is split into three peaks with 1:2:1 relative intensities. The methylene protons appear as a quartet, with the relative intensities of 1:3:3:1.
Qualitatively, any spin plus-half nucleus polarizes the spins of its electrons to the minus-half state. Consequently, the paired electron in the hydrogen–carbon bond must...
1.4K
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule

2.3K
In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the...
2.3K

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Related Experiment Video

Updated: Dec 22, 2025

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

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Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy.

Arnab Chakraborty1, Fabien Deligey1, Jenny Quach1

  • 1Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, U.S.A.

Biochemical Society Transactions
|May 8, 2020
PubMed
Summary
This summary is machine-generated.

Magic-angle spinning dynamic nuclear polarization (MAS-DNP) enhances solid-state nuclear magnetic resonance (ssNMR) for studying biomolecules. This technique reveals atomic-level details of cellular processes, aiding biomaterial and drug development.

Keywords:
cell walldynamic nuclear polarizationmembrane proteinspathogenic fungipolysaccharidessolid-state NMR

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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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Area of Science:

  • Biophysics
  • Structural Biology
  • Biomolecular NMR Spectroscopy

Background:

  • Solid-state nuclear magnetic resonance (ssNMR) is crucial for analyzing insoluble biomolecules.
  • Limitations in sensitivity and scope have historically hindered ssNMR applications.
  • Advancements are needed to probe complex biological systems at high resolution.

Purpose of the Study:

  • To highlight emerging magic-angle spinning dynamic nuclear polarization (MAS-DNP) techniques.
  • To showcase MAS-DNP applications in biomolecular composites and intact cells.
  • To demonstrate the utility of MAS-DNP in understanding protein folding, ligand binding, and biopolymer polymorphism.

Main Methods:

  • Application of sensitivity-enhanced ssNMR using MAS-DNP.
  • Analysis of polymer interfaces within cellular environments.
  • Investigation of interactions between carbohydrates, proteins, and lignin.

Main Results:

  • MAS-DNP enables detection of polymer interfaces in cellular settings.
  • Detailed structural and dynamic information obtained for proteins and biopolymers.
  • Insights into physical interactions within complex biomolecular systems.

Conclusions:

  • MAS-DNP significantly expands the capabilities of ssNMR for biomolecular research.
  • Provides atomic-level understanding of cellular processes and biomolecular interactions.
  • Promotes development of novel biomaterials and therapeutic inhibitors.