Related Experiment Video
Updated: Dec 22, 2025
10:40
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
Published on: April 25, 2022
2.7K
RNA Editing Alters miRNA Function in Chronic Lymphocytic Leukemia
Franz J Gassner1, Nadja Zaborsky1, Daniel Feldbacher1,2
1Department of Internal Medicine III with Haematology, Medical Oncology, Haemostaseology, Infectiology and Rheumatology, Oncologic Center, Salzburg Cancer Research Institute-Laboratory for Immunological and Molecular Cancer Research (SCRI-LIMCR), Paracelsus Medical University, Cancer Cluster Salzburg, Müllner Hauptstrasse 48, 5020 Salzburg, Austria.
Cancers
|May 9, 2020
Summary
Researchers discovered specific RNA editing patterns in microRNAs (miRNAs) of chronic lymphocytic leukemia (CLL) patients. This finding sheds light on the role of miRNA editing in CLL pathogenesis and disease progression.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Chronic lymphocytic leukemia (CLL) is a common B cell leukemia with variable outcomes.
- MicroRNAs (miRNAs) are key regulators of gene expression implicated in CLL.
- The role of RNA editing in miRNA diversification in CLL remains unexplored.
Purpose of the Study:
- To map adenosine to inosine (A-to-I) and cytosine to uracil (C-to-U) RNA editing in miRNAs from CLL and normal B cells.
- To identify specific miRNA editing patterns associated with CLL.
- To investigate the potential role of miRNA editing in CLL pathogenesis.
Main Methods:
- Analysis of next-generation sequencing data.
- Comparative analysis of miRNA editing in CLL versus normal B cells.
- Identification and characterization of RNA editing events in specific miRNAs.
Main Results:
- Detailed map of A-to-I and C-to-U editing in miRNAs from CLL and normal B cells.
- Identification of CLL-specific miRNA expression patterns.
- Discovery of specific RNA editing in numerous miRNAs, notably miR-3157 and miR-6503, in CLL patients.
Conclusions:
- RNA editing contributes to miRNA diversification in CLL.
- Specific miRNA editing patterns are associated with chronic lymphocytic leukemia.
- MiRNA editing may play a significant role in the pathogenesis of CLL.
More Related Videos
Related Concept Videos
02:23RNA Editing
9.6K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.6K
01:22MicroRNAs
3.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
01:22MicroRNAs
23.7K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.7K
01:32RNA Splicing
60.1K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.1K
01:20Mismatch Repair
6.1K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
6.1K
02:41Chromatin Structure Regulates pre-mRNA Processing
8.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
8.0K