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Circulating CD14+HLA-DR-/low Myeloid-Derived Suppressor Cells as Potential Biomarkers for the Identification of

Shipeng Sun1, Yali Wei2, Xue Zeng2

  • 1Clinical Laboratories, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Evidence-Based Complementary and Alternative Medicine : Ecam
|May 9, 2020
PubMed

Insights

Researchers identified potential biomarkers for Traditional Chinese Medicine (TCM) syndromes in psoriasis. Increased Mo-MDSCs and specific mRNA levels may help differentiate blood-heat and blood-stasis syndromes in psoriasis patients.

Area of Science:

  • Immunology
  • Dermatology
  • Traditional Chinese Medicine (TCM)

Background:

  • Psoriasis is a chronic autoimmune disease requiring better diagnostic tools.
  • Traditional Chinese Medicine (TCM) classifies psoriasis into distinct syndromes, such as blood-heat (BH) and blood-stasis (BS).
  • Identifying biomarkers for these TCM syndromes can aid in understanding disease heterogeneity and developing targeted therapies.

Purpose of the Study:

  • To investigate circulating monocytic myeloid-derived suppressor cells (Mo-MDSCs) and associated immune factors in psoriasis patients.
  • To identify potential biomarkers for differentiating between TCM blood-heat (BH) and blood-stasis (BS) syndromes in psoriasis.

Main Methods:

  • Analysis of Mo-MDSC frequency (CD14+HLA-DR-/low cells within CD14+ cells) in peripheral blood.
  • Quantification of serum immune factors (cytokines and enzymes).
  • Measurement of mRNA expression levels of key immune genes in peripheral blood mononuclear cells (PBMCs).

Main Results:

  • Mo-MDSC frequency was significantly higher in plaque psoriasis patients with blood-stasis (BS) syndrome compared to healthy controls and blood-heat (BH) syndrome patients.
  • Serum levels of several cytokines (IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-α, IFN-γ) and enzymes (iNOS, Arg-1) did not differ significantly between groups.
  • Increased mRNA expression of Arg-1, TNF-α, ROR-γ, and PD-L1, and decreased mRNA expression of PD-1 and IL-10 were observed in PBMCs of psoriasis patients compared to controls.
  • Distinct mRNA expression patterns for TNF-α and FOXP3 were found between the BH and BS syndrome groups.

Conclusions:

  • The percentage of CD14+HLA-DR-/low Mo-MDSCs within CD14+ cells is a potential biomarker for distinguishing psoriasis TCM syndromes.
  • Differential mRNA expression of TNF-α and FOXP3 in PBMCs may serve as biomarkers for differentiating between TCM BH and BS syndromes in psoriasis.

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