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Related Concept Videos

RNA Structure01:19

RNA Structure

6.6K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
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RNA Structure01:23

RNA Structure

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Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K
Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Related Experiment Video

Updated: Dec 22, 2025

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

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RSVdb: a comprehensive database of transcriptome RNA structure.

Haopeng Yu, Yi Zhang, Qing Sun

    Briefings in Bioinformatics
    |May 9, 2020
    PubMed
    Summary
    This summary is machine-generated.

    A new database, RSVdb, helps researchers visualize and analyze RNA structures across entire transcriptomes. This resource aids in understanding RNA

    Keywords:
    MaP methodRNA structure predictionRSVdbRT-stop methoddimethyl sulfate probingtranscriptome RNA structure database

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    Area of Science:

    • Molecular Biology
    • Bioinformatics
    • Genomics

    Background:

    • RNA molecules fold into complex structures essential for cellular regulation.
    • Dimethyl sulfate (DMS) is a chemical probe used to map RNA structures at the transcriptome level.

    Purpose of the Study:

    • To introduce RSVdb, a comprehensive database for browsing and visualizing transcriptome-wide RNA structures.
    • To provide a centralized resource for RNA structure data and analysis tools.

    Main Methods:

    • Compilation of 626,225 RNA structures with validated DMS reactivity from 178 samples across eight species.
    • Development of a web-based platform supporting information retrieval, research overview, structure prediction, and data download.

    Main Results:

    • RSVdb offers functionalities for searching species, studies, transcripts, and genes.
    • The database includes quality control of sequencing data, statistical charts of RNA structure information, and in silico structure prediction tools.

    Conclusions:

    • RSVdb serves as a valuable reference for RNA structure, facilitating research into RNA structure-function relationships.
    • The database supports future investigations of RNA structure's role at the transcriptome level.