Bacillus dafuensis sp. Nov., Isolated from a Forest Soil in China

A Gram-staining-positive, motile, rod-shaped, aerobic bacterial strain FJAT-25496 ^T was isolated from a forest soil sample collected from Dafu County, Chengdu City, Sichuan Province, China. Strain FJAT-25496 ^T grew at 15-50 °C (optimum 35 °C), pH 6.0-10.0 (optimum 8.0), and NaCl tolerance of 0-4.0% (w/v) (optimum 0%). The strain contained iso-C_15:0 and anteiso-C_15:0 as the predominant cellular fatty acids as well as MK-7 as the only menaquinone. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and phosphatidyl glycerol (PG). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FJAT-25496 ^T was a member of the genus Bacillus, and most closely related to Bacillus solani FJAT-18043 ^T (97.8%), Bacillus praedii FJAT-25547 ^T (97.8%), Bacillus depressus BZ1^T (97.7%), Bacillus oceanisediminis H2^T (97.7%), and Bacillus ciccensis 5L6^T (97.6%). Based on complete genome comparisons between strain FJAT-25496 ^T and the type strains of its closely related species, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were 73.4-76.3% and 21.7-23.2%, respectively. The genomic DNA G + C content of strain FJAT-25496 ^T was 36.9%. On the basis of phenotypic, phylogenetic, chemotaxonomic, and genomic features, strain FJAT-25496 ^T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus dafuensis sp. nov. is proposed. The type strain is FJAT-25496 ^T (= CCTCC AB 2019182 ^T = KCTC 43120 ^T).