Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.4K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.4K
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

41.3K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
41.3K
Global Regulatory Systems01:28

Global Regulatory Systems

475
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
475
Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response01:15

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

282
Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...
282
Regulation of Metabolism01:19

Regulation of Metabolism

11.2K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
11.2K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.1K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A new perspective on ATR's role in translesion synthesis.

Genes & development·2026
Same author

Circadian Controlled Transcription in Brain and Peripheral Organs of Juvenile and Adult Mice.

International journal of molecular sciences·2026
Same author

Depression of tryptophan may contribute to adverse effects of naproxen.

Nature communications·2026
Same author

Age and the Diurnal Oscillatory Features of the Human Chronobiome.

medRxiv : the preprint server for health sciences·2026
Same author

Concomitant COX-1 and COX-2 suppression is not sufficient to induce enteropathy associated with chronic NSAID use.

The Journal of clinical investigation·2026
Same author

Combinatorial treatment of glioblastoma with temozolomide (TMZ) plus 5-ethynyl-2'-deoxyuridine (EdU).

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

The effects of two Leu-to-Pro substitutions, L57P and L43P, on structural and functional properties of cardiac tropomyosin.

The FEBS journal·2026
Same journal

Stimulating proteasomal degradation in human proteinopathies.

The FEBS journal·2026
Same journal

A lipid-sensitive food choice behavior influences aging outcomes from a longevity-promoting diet.

The FEBS journal·2026
Same journal

The interaction network of a rice seed-specific transcription factor OsMADS29 and the calcium sensors, calmodulin, and calmodulin-like proteins.

The FEBS journal·2026
Same journal

A large family of unusual voltage-sensing proton channels (Hv3) in mollusks.

The FEBS journal·2026
Same journal

RVB-1 and RVB-2 are stress responsive proteins in Neurospora crassa and RVB-1 interacts with the centromeric Shugoshin (SGO-1) protein.

The FEBS journal·2026
See all related articles

Related Experiment Video

Updated: Dec 22, 2025

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
07:42

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

13.2K

CRY1-CBS binding regulates circadian clock function and metabolism.

Sibel Cal-Kayitmazbatir1, Eylem Kulkoyluoglu-Cotul2, Jacqueline Growe3

  • 1Department of Molecular Biology and Genetics, Koc University, Istanbul, Turkey.

The FEBS Journal
|May 9, 2020
PubMed
Summary
This summary is machine-generated.

The enzyme cystathionine β-synthase (CBS) interacts with cryptochrome 1 (CRY1), influencing circadian rhythms and metabolism. This interaction acts as a switch, modulating cellular physiology and metabolic control.

Keywords:
circadian rhythmcryptochromecystathionine β-synthasemetabolismtranscriptional regulation

More Related Videos

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

8.7K
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

10.3K

Related Experiment Videos

Last Updated: Dec 22, 2025

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
07:42

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

13.2K
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

8.7K
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

10.3K

Area of Science:

  • Biochemistry
  • Chronobiology
  • Metabolomics

Background:

  • Circadian disruption negatively impacts metabolic health, yet the underlying mechanisms remain unclear.
  • Metabolism and circadian rhythms are known to influence each other.
  • Understanding the molecular links between these systems is crucial for metabolic health.

Purpose of the Study:

  • To investigate the functional interaction between cystathionine β-synthase (CBS) and cryptochrome 1 (CRY1).
  • To elucidate the role of this interaction in regulating circadian rhythms and metabolic pathways.
  • To determine the impact of mutations in CBS on its interaction with CRY1 and circadian function.

Main Methods:

  • Cellular assays to assess CBS-CRY1 interaction and CRY1 repressor activity.
  • Generation and analysis of transgenic CbsZn/Zn mice.
  • Assessment of circadian locomotor activity in mice.
  • Enzymatic activity assays of CBS in liver extracts from wild-type and mutant mice.
  • Metabolomic analysis of various mouse models (WT, CbsZn/Zn, Cry1-/-, Cry2-/-).

Main Results:

  • CBS functionally interacts with CRY1, enhancing CRY1-mediated repression of the CLOCK/BMAL1 complex and shortening circadian period.
  • The common homocystinuria-associated mutant CBS-I278T fails to bind CRY1 or regulate its activity.
  • CbsZn/Zn mice show reduced circadian power and altered E-BOX output despite normal circadian period.
  • CRY1 binding reciprocally modulates CBS enzymatic activity; Cry1-/- liver extracts exhibit reduced CBS activity.
  • Metabolomic analyses reveal CRY1-dependent temporal variations in one-carbon and transsulfuration pathways.

Conclusions:

  • CBS-CRY1 binding represents a novel post-translational regulatory mechanism.
  • This interaction serves as a crucial switch modulating cellular circadian physiology and metabolic control.
  • Disruption of the CBS-CRY1 interaction, as seen in homocystinuria, may have significant metabolic consequences.