Potential concerns in fullerene application to water treatment related to transformation, cellular uptake and...

Fullerene (C₆₀) exhibits versatile properties that shows great potential for improving water treatment technologies. However, the probable transformation of C₆₀ during water treatment, which consequently changes the physicochemical properties and toxicity of the parent compound, may introduce doubt concerning its application. Our results demonstrated that the C₆₀ aggregate (nC₆₀) was transformed to a more oxidized form under common water disinfection processes (i.e., ultraviolet irradiation and photochlorination). The light-irradiated product (UV_nC₆₀) exhibited lower cytotoxicity toward macrophage J774A.1 cells relative to nC₆₀, whereas the photochlorinated product (UV/Cl_nC₆₀) increased the toxic effect. Particularly, the internalization of nanoparticles and the mimetic superoxide dismutase (SOD) activity resulted in the selective accumulation of intracellular hydrogen peroxide. Thus, sequential exposure to a nonlethal dose of nanoparticles followed by 5 μM copper ions (which is a much lower level than the EPA-regulated level of 20 μM in drinking water) led to the significant production of hydroxyl radicals inside cells. The uptake and SOD-like activity were highly structure-related, with the most noteworthy activity obtained for UV/Cl_nC₆₀. These results emphasize that environmental transformation-induced property changes should be given adequate consideration in the risk assessment of C₆₀.