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Updated: Dec 21, 2025
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
Investigation of endogenous malondialdehyde through fluorescent probe MDA-6 during oxidative stress
Jing Zhang1, Zihao Yang2, Shanshan Zhang1
1Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832002, China.
Abstract:
The malondialdehyde (MDA)-specific detection probe (MDA-6) was successfully synthesised through the photoinduced electron transfer (PET) mechanism which possesses many biological applications. In vivo biological applicability of this probe was proved in different cell lines, zebrafish and mice. In these models, the MDA was produced by oxygen stress injury and the relationship between MDA and probe were evaluated in vitro as well as in vivo under different stress conditions. After comparing evaluated results with commercial MDA kit, MDA-6 was concluded with high specificity, low limit of detection (0.03 μM), and can achieve micro-detection of MDA with low cytotoxicity, demonstrating MDA-6 enables safe and effective detection.
Insights
A novel malondialdehyde (MDA)-specific probe, MDA-6, was synthesized and validated for biological applications. This probe offers sensitive, specific, and safe detection of MDA in various biological models.
Area of Science:
- Biochemistry
- Chemical Biology
- Molecular Imaging
Background:
- Malondialdehyde (MDA) is a biomarker for oxidative stress.
- Existing MDA detection methods have limitations in specificity and sensitivity.
- Development of targeted probes for MDA is crucial for biological research.
Purpose of the Study:
- To synthesize and characterize a novel MDA-specific detection probe, MDA-6.
- To evaluate the in vitro and in vivo applicability of MDA-6 for detecting MDA.
- To assess the performance of MDA-6 compared to commercial MDA detection kits.
Main Methods:
- Synthesis of MDA-6 probe via photoinduced electron transfer (PET) mechanism.
- In vitro and in vivo evaluation in cell lines, zebrafish, and mice.
- Induction of oxidative stress to generate MDA.
- Comparison with a commercial MDA detection kit.
Main Results:
- Successful synthesis of MDA-6 probe.
- Demonstrated in vivo applicability in cell lines, zebrafish, and mice.
- High specificity and low limit of detection (0.03 μM) for MDA.
- Effective micro-detection of MDA with low cytotoxicity.
- Comparable or superior performance to commercial MDA kits.
Conclusions:
- MDA-6 is a highly specific and sensitive probe for malondialdehyde detection.
- The probe exhibits excellent biological applicability in various models.
- MDA-6 offers a safe and effective tool for micro-detection of MDA in biological research.
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