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DNA-based microarray studies in visceral leishmaniasis: identification of biomarkers for diagnostic, prognostic and
Awanish Kumar1, Satish Chandra Pandey2, Mukesh Samant3
1Department of Biotechnology, National Institute of Technology, Raipur (Chhattisgarh), INDIA.
Abstract:
Visceral leishmaniasis (VL) is one of the major infectious diseases affecting the poorest regions of the world. Current therapy is not very much satisfactory. The alarming rise of drug resistance and the unavailability of an effective vaccine against VL urges research towards identifying new targets or biomarkers for its effective treatment. New technology developments offer some fresh hope in its diagnosis, treatment, and control. DNA microarray approach is now broadly used in parasitology research to facilitate the thoughtful of mechanisms of disease and identification of drug targets and biomarkers for diagnostic and therapeutic development. An electronic search on "VL" and "Microarray" was conducted in Medline and Scopus and papers published in the English mentioning use of DNA microarray on VL were selected and read to write this paper review. Functional analysis and interpretation of microarray results remain very challenging due to the inherent nature of experimental workflows, access, cost, and complexity of data obtained. We have explained and emphasized the use of curate knowledge of microarray in the case of VL for the identification of therapeutic target and biomarker and their selection/implementation in clinical use.
Insights
Visceral leishmaniasis (VL) research benefits from DNA microarray technology for identifying new drug targets and biomarkers. This review emphasizes curated knowledge to overcome data challenges for improved VL diagnosis and treatment.
Area of Science:
- Parasitology
- Infectious Diseases
- Biotechnology
Background:
- Visceral leishmaniasis (VL) is a significant global health issue, particularly in impoverished areas.
- Current treatments for VL are suboptimal, with rising drug resistance and no available vaccine.
- Novel diagnostic and therapeutic strategies are urgently needed for effective VL control.
Purpose of the Study:
- To review the application of DNA microarray technology in visceral leishmaniasis research.
- To highlight the potential of DNA microarrays in identifying drug targets and biomarkers for VL.
- To discuss the challenges and strategies for interpreting microarray data in the context of VL.
Main Methods:
- An electronic literature search was performed on Medline and Scopus using keywords 'VL' and 'Microarray'.
- Selected English-language papers detailing DNA microarray use in VL were reviewed.
- Functional analysis and interpretation of microarray data were discussed.
Main Results:
- DNA microarrays offer a powerful tool for understanding VL disease mechanisms.
- Microarray analysis can facilitate the identification of potential therapeutic targets and diagnostic biomarkers for VL.
- Challenges in data interpretation require curated knowledge and robust analytical approaches.
Conclusions:
- DNA microarray technology holds significant promise for advancing VL research.
- Effective utilization of curated microarray data can aid in the selection and clinical implementation of novel therapeutic targets and biomarkers.
- Further development in data analysis is crucial for maximizing the impact of microarrays in combating VL.
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