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Updated: Dec 21, 2025
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Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
Published on: March 20, 2018
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Parametric mapping of cellular morphology in plant tissue sections by gray level granulometry
David Legland1, Fabienne Guillon1, Marie-Françoise Devaux1
1UR1268 Biopolymères, Interactions et Assemblages, INRAE, Nantes, France.
Plant Methods
|May 12, 2020
Summary
This study introduces a new method to map cellular morphology in plant tissues using image texture analysis. This approach quantifies variations in cell size and shape across plant organs, aiding in understanding genotype and environmental influences.
Area of Science:
- Plant biology
- Quantitative histology
- Image analysis
Background:
- Cellular morphology influences physical properties of plant organs.
- Quantifying cellular morphology variations within tissues is challenging.
- Image texture analysis is effective for plant histology at the image scale.
Purpose of the Study:
- To develop a method for parametric mapping of cellular morphology in plant tissue images.
- To quantify variations in cellular morphology within plant organs.
Main Methods:
- Utilizes gray level granulometry from mathematical morphology for texture feature extraction.
- Employs Centroidal Voronoi Diagram for image partitioning.
- Interprets granulometric curves via multivariate data analysis or summary features for local cell size.
Main Results:
- Generates parametric maps detailing cellular morphology variations within plant tissue images.
- Provides a quantitative description of local average cell size variations.
Conclusions:
- Proposes a methodology for quantifying cellular morphology and its variations in tissue sections.
- Aids in understanding genotype/environment effects on cellular morphology.
- Clarifies relationships between cellular morphology and cell wall chemical composition.
