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A Modified Precipitation Method to Isolate Urinary Exosomes
Published on: January 16, 2015
[Diagnostic value of molecular weight related urinary protein electrophoresis in normal total protein excretion]
1Klinik und Poliklinik für Innere Medizin, Martin-Luther-Universität Halle-Wittenberg.
Abstract:
The electrophoresis of urinary proteins related to the molecular weight in SDS-polyacrylamide gels stood the test for the clarification of the cause of proteinurias. Also in normal excretion of the total protein the analysis of protein is reasonable after concentration of the urine. In the electrophoretic separation of 104 urinary specimens with a maximum protein content of 0.15 g/l in 44 cases a normal protein pattern was found. In 60 cases pathological patterns occurred which in their protein composition greatly corresponded to the damage within the nephron to be expected in the different clinical diagnoses. The results show that the SDS-polyacryl amide gel electrophoresis is suitable for the early recognition and differentiation of injuries of the kidneys.
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