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Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
Published on: August 29, 2018
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Comparative Transcriptome Landscape of Mouse and Human Hearts.
Tatsuya Anzai1,2, Takanori Yamagata2, Hideki Uosaki1
1Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan.
Frontiers in Cell and Developmental Biology
|May 12, 2020
Summary
This study reveals significant differences in organ development between mice and humans, finding that mouse hearts at P0-3 and human hearts at 18-19 weeks post-conception (wpc) show the most transcriptomic similarity. Novel maturation markers were identified, emphasizing the need for human-centric research in cardiomyocyte development.
Area of Science:
- Developmental Biology
- Comparative Genomics
- Cardiovascular Research
Background:
- Organ development and maturation exhibit species-specific trajectories.
- Transcriptome analysis provides insights into cellular and organogenesis processes.
- Understanding cardiomyocyte maturation is crucial for cardiac regenerative medicine.
Purpose of the Study:
- To reanalyze mouse and human organ transcriptomes for a deeper understanding of human cardiomyocyte maturation.
- To identify species-conserved maturation markers for cardiomyocytes.
- To evaluate the maturation state of human pluripotent stem cell-derived cardiomyocytes (PSC-CMs).
Main Methods:
- Comparative transcriptome analysis of mouse and human organ datasets.
- Identification and validation of novel gene markers for cardiac maturation.
- Assessment of PSC-CM maturation against human fetal and adult heart transcriptomes.
Main Results:
- Significant differences in ribosomal gene expression between developing mouse and human organs were identified.
- Transcriptomic proximity between mouse hearts (P0-3) and human hearts (18-19 wpc) was observed.
- Novel maturation marker genes with higher cross-species consistency were discovered, outperforming conventional markers in human samples.
- Human PSC-CMs exhibited limited expression of key maturation markers compared to in vivo human cardiac tissues.
Conclusions:
- Mouse and human organ developmental timelines, particularly for the heart, do not directly correspond.
- Novel, conserved markers are essential for accurately assessing human cardiomyocyte maturation.
- Current human PSC-CM maturation protocols require enhancement, highlighting the limitations of mouse models for studying human cardiac development.
