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Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
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Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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U-Shaped Mobility Analyzer: A Compact and High-Resolution Counter-Flow Ion Mobility Spectrometer.

Keke Wang1, Ran Qiu1, Xiaoqiang Zhang1

  • 1Shimadzu Research Laboratory (Shanghai) Co. Ltd., Shanghai 201206, People's Republic of China.

Analytical Chemistry
|May 12, 2020
PubMed
Summary

A new U-shaped mobility analyzer (UMA) enhances ion mobility spectrometry-mass spectrometry (IMS-MS) with high-resolution filtering. This device improves dynamic range for analyzing complex samples and low-concentration analytes in omics studies.

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Area of Science:

  • Analytical Chemistry
  • Spectrometry
  • Biophysics

Background:

  • Counter-flow ion mobility spectrometers (IMS) offer improved resolution and functionality.
  • There is a growing need for advanced analytical techniques in omics research.

Purpose of the Study:

  • To develop a novel U-shaped mobility analyzer (UMA) for enhanced ion mobility spectrometry-mass spectrometry (IMS-MS).
  • To demonstrate the UMA's capability for high-resolution ion selection and improved dynamic range.

Main Methods:

  • Development of a U-shaped mobility analyzer (UMA) with two channels for ion manipulation.
  • Implementation of a mobility band-pass filter by controlling electric fields in the UMA channels.
  • Operation in both filter-scan and trap-scan modes for ion analysis.

Main Results:

  • Achieved a resolution of ~180 for small organic molecules and ~370 for myoglobin in filter-scan mode.
  • Achieved higher resolutions of ~210 for small organic molecules and ~590 for myoglobin in trap-scan mode.
  • Demonstrated significant enhancement in the dynamic range of IMS-MS instruments.

Conclusions:

  • The UMA device offers high resolution and dynamic range, benefiting targeted analysis in complex matrices.
  • The UMA is suitable for omics studies, particularly for analyzing low-concentration analytes in high chemical backgrounds.
  • The UMA's filter-scan mode is advantageous for scrutinizing trace analytes.