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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Electrochemical SERS on 2D Mapping for Metabolites Detection.

Yow-Kuan Lin1, Ruo-Ju Tai1, Shu-Chen Wei2

  • 1Department of Materials Science and Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.

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|May 12, 2020
PubMed
Summary

This study presents a new electrochemical surface-enhanced Raman scattering (EC-SERS) method with optimized mapping for accurate quantification of low-concentration analytes. The technique successfully quantified azathioprine metabolites, 6-thioguanine nucleotides (6-TGNs) and 6-methylmercaptopurine (6-MMP), achieving low detection limits.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Materials Science

Background:

  • Surface-enhanced Raman scattering (SERS) offers high sensitivity for bioanalysis but faces challenges in accurate quantification, especially at low concentrations.
  • Clinical applications of 2D SERS-active substrates are hindered by difficulties in achieving reliable quantitative results.

Purpose of the Study:

  • To develop an analytical method integrating optimized sample mapping with electrochemical SERS (EC-SERS) for accurate quantification.
  • To address the challenge of low-concentration analyte detection and quantification in bioanalysis.
  • To demonstrate the method's efficacy using azathioprine metabolites, 6-thioguanine nucleotides (6-TGNs) and 6-methylmercaptopurine (6-MMP).

Main Methods:

  • Fabrication of a conductive SERS-active substrate via electrochemical deposition of gold nanoparticles (AuNPs) on indium tin oxide glass.
  • Application of a negative potential to enhance Raman intensity through charge transfer and reorientation, forming Au-S bonds.
  • Optimization of sample mapping range to reduce SERS intensity standard deviation to below 10%.

Main Results:

  • Achieved significant Raman intensity enhancement for 6-TGNs and 6-MMP upon applying a negative potential.
  • Demonstrated quantitative analysis with detection limits of 10 nM for 6-TGNs and 100 nM for 6-MMP.
  • Reduced SERS intensity standard deviation to consistently below 10% through optimized mapping.

Conclusions:

  • The integrated EC-SERS and mapping strategy provides a reliable and quantitative analytical platform for electrochemically modifiable analytes.
  • This method overcomes limitations in SERS quantification, enabling sensitive detection of important biomolecules.
  • The developed technique holds promise for clinical applications requiring precise quantification of ultralow concentration analytes.