Marinicella rhabdoformis sp. nov., isolated from coastal sediment

A Gram-stain-negative, rod-shaped, facultative anaerobic bacterium, designated strain 3539^T, was isolated from coastal sediment of Weihai, PR China. Optimal growth occurred at 28 °C, pH 7.5-8.0 and in the presence of 3.0 % (w/v) NaCl. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 3539^T formed a robust clade with members of the genus Marinicella and was closely related to Marinicella litoralis JCM 16154^T, Marinicella sediminis F2^T and Marinicella pacifica sw153^T with 97.7, 96.2 and 95.4 % sequence similarity, respectively. The average amino acid identity, percentage of conserved proteins, average nucleotide identity and digital DNA-DNA hybridization values between strain 3539^T and M. litoralis JCM 16154^T were 64.9, 68.3, 72.8 and 18.9 %, respectively. The genomic DNA G+C content of strain 3539^T was 42.0 mol%. The dominant respiratory quinone was ubiquinone-8, and the major fatty acids were iso-C_15 : 0 and summed feature 3 (C_16 : 1  ω7c/C_16 : 1  ω6c). The polar lipids of strain 3539^T consisted of phosphatidyldimethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified aminophospholipid, one unidentified lipid and three unidentified phospholipids. Based on the combination of phylogenetic, phenotypic and chemotaxonomic data, strain 3539^T is considered to represent a novel species within the genus Marinicella in he family Alcanivoracaceae, for which the name Marinicella rhabdoformis sp. nov. is proposed. The type strain of the new species is 3539^T (=KCTC 72414^T=MCCC 1H00388^T).