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Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR Flow Cytometry

Maayan Schwarzkopf1, Harry M T Choi1, Niles A Pierce2,3,4

  • 1Division of Biology & Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2020
PubMed

Keywords:
AccuracyAutofluorescenceAutomatic background suppressionBacterial cellsFluorescence in situ hybridization (FISH)High-throughput expression profilingHybridization chain reaction (HCR)In situ HCR v3.0Mammalian cellsMultiplexedPrecisionQuantitativeSignal amplificationmRNA flow cytometryqHCR flow cytometry

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