Nanoliposome Precursors for Shape Modulation: Use of Heuristic Algorithm and QBD Principles for Encapsulating...

Area of Science:

• Pharmaceutical Sciences
• Nanotechnology
• Drug Delivery Systems

Background:

• Method screening for nanoliposome (NL) development is inefficient, leading to material loss, time wastage, and increased costs.
• A rapid and reliable decision-making process is essential for formulators.
• Optimizing NL fabrication is key for advanced nanoformulations.

Purpose of the Study:

• To systematically select the optimal method for developing nanoliposomes (NL) using the Analytic Hierarchy Process (AHP).
• To investigate curcumin-loaded NL fabrication.
• To establish a precursor for nanocochleates.

Main Methods:

• Analytic Hierarchy Process (AHP) with pair-wise comparison matrices to determine the priority weight and ranking of fabrication methods.
• Plackett-Burman Design (PBD) to assess the impact of formulation and process variables on particle size, drug content, and entrapment efficiency.
• Validation of AHP results using Bland-Altman plots and control charts.

Main Results:

• The ethanol injection method was identified as the optimal NL fabrication technique with the highest priority weight (0.337) via AHP.
• PBD indicated that stirring speed, phospholipid amount, and cholesterol significantly influenced particle size and entrapment efficiency (p<0.05).
• Successful preparation of NL with desired particle size and distribution was achieved.

Conclusions:

• AHP provides an effective decision-making tool for selecting optimal nanoliposome fabrication methods.
• PBD elucidates the influence of formulation variables, guiding further optimization.
• Nanoliposomes are promising candidates for developing tailored nanoformulations for in vitro and in vivo applications.