Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temperature-Dependent Conformational Dynamics of Substrate Entrance Loops in β-Glucosidase: Insights from Molecular Dynamics Simulations.

International journal of molecular sciences·2026
Same author

Temperature-Dependent Active-Site Rearrangements of PETaseSM14: Insights from Molecular Dynamics Simulations.

International journal of molecular sciences·2026
Same author

Evaluation of AI-Predicted GH11 Xylanase Models Against a Previously Unreported Experimental Structure: Implications for Conformational Accuracy and Ligand Binding.

International journal of molecular sciences·2026
Same author

Special Issue "Structural Dynamics of Macromolecules".

International journal of molecular sciences·2026
Same author

Structural flexibility of the nicotinamide group of NADH in butanol dehydrogenase YqdH from Fusobacterium nucleatum.

PloS one·2025
Same author

Structure-Based Understanding of Cu<sup>2+</sup> Coordination in Fluorescent Proteins for Metal Biosensor Applications-A Review.

Biosensors·2025

Related Experiment Video

Updated: Dec 21, 2025

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
07:28

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Published on: September 23, 2020

3.5K

Polysaccharide-Based Injection Matrix for Serial Crystallography.

Ki Hyun Nam1

  • 1Department of Life Science, Pohang University of Science and Technology, Pohang 37673, Korea.

International Journal of Molecular Sciences
|May 14, 2020
PubMed
Summary

New polysaccharide-based media, wheat starch (WS) and alginate, enable stable sample delivery for serial crystallography (SX). These novel media facilitate room-temperature macromolecular structure determination, expanding SX applications.

Keywords:
SFXSMXalginatebackground scatteringsample deliveryserial crystallographyviscous mediumwheat starch

More Related Videos

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

9.4K
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
14:04

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Published on: January 16, 2021

4.9K

Related Experiment Videos

Last Updated: Dec 21, 2025

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
07:28

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Published on: September 23, 2020

3.5K
Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

9.4K
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
14:04

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Published on: January 16, 2021

4.9K

Area of Science:

  • Structural Biology
  • Biophysics
  • Materials Science

Background:

  • Serial crystallography (SX) allows room-temperature observation of molecular dynamics.
  • Current viscous media for crystal delivery in SX can chemically or biologically interact with samples.
  • Novel delivery media are needed to expand SX applications.

Purpose of the Study:

  • To prepare and characterize new polysaccharide-based sample delivery media for SX.
  • To evaluate the suitability of wheat starch (WS) and alginate for crystal delivery.

Main Methods:

  • Preparation and characterization of WS and alginate-based injection media.
  • Assessment of injection stream stability at flow rates below 200 nL/min.
  • Serial millisecond crystallography (SMX) experiments at X-ray facilities.
  • Determination of room-temperature crystal structures using the new media.

Main Results:

  • WS and alginate media demonstrated stable injection streams.
  • Low X-ray background scattering was observed, comparable to existing hydrogels.
  • Room-temperature crystal structures of glucose isomerase and lysozyme were determined at 1.9-2.0 Å resolution.

Conclusions:

  • Wheat starch and alginate are effective and versatile media for SX sample delivery.
  • These new media enhance the applicability of SX for studying macromolecular dynamics.
  • The development broadens the range of available materials for SX experiments.