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Effects of T-2 mycotoxin on tumor susceptibility in mice
1USDA, Veterinary Toxicology and Entomology Research Laboratory, College Station, TX 77841.
Abstract:
The effect of Fusarium-produced T-2 toxin on tumor growth was evaluated in ICR, CFW, and C57B6/6 mice inoculated with murine sarcoma, Ehrlich ascites carcinoma, or B16F1 melanoma tumor cell lines. Mice were given T-2 toxin intragastrically either at the rate of 2 mg of toxin/kg of body weight daily for 5 days or a single dosage of 4 mg of toxin/kg and were inoculated SC with tumor cells 1 or 2 days after administration of toxin. Tumor growth was assessed 15 to 41 days after tumor challenge by determining the frequency of tumor development and tumor weights. Significant increases in the frequency of development of murine sarcoma (P less than 0.005), Ehrlich ascites carcinoma (P less than 0.01), and B16F1 melanoma tumors (P less than 0.05) were detected in toxin-treated mice, compared with control mice. Murine sarcoma and B16F1 melanoma tumor weights also were significantly (P less than 0.01) higher in toxin-treated mice. The effect of T-2 toxin on tumor growth was more marked after 5 daily treatments than after a single dose.
Insights
Fusarium T-2 toxin significantly increased tumor development and weight in mice across multiple cancer types. This T-2 toxin effect on tumor growth was more pronounced with repeated daily doses compared to a single dose.
Area of Science:
- Toxicology
- Oncology
- Immunology
Background:
- T-2 toxin is a trichothecene mycotoxin produced by Fusarium species.
- Mycotoxins can have various biological effects, including immunosuppression and cytotoxicity.
- The impact of T-2 toxin on tumor progression requires further investigation.
Purpose of the Study:
- To evaluate the effect of Fusarium-produced T-2 toxin on tumor growth in different mouse models.
- To compare the impact of single versus multiple doses of T-2 toxin on tumor development and weight.
Main Methods:
- ICR, CFW, and C57B6/6 mice were inoculated with murine sarcoma, Ehrlich ascites carcinoma, or B16F1 melanoma.
- Mice received T-2 toxin intragastrically at 2 mg/kg daily for 5 days or a single dose of 4 mg/kg.
- Tumor incidence and weight were assessed 15–41 days post-inoculation.
Main Results:
- T-2 toxin significantly increased tumor development frequency for all tested tumor types (murine sarcoma, Ehrlich ascites carcinoma, B16F1 melanoma).
- T-2 toxin significantly increased tumor weights for murine sarcoma and B16F1 melanoma.
- A more pronounced effect on tumor growth was observed after 5 daily T-2 toxin treatments compared to a single dose.
Conclusions:
- Fusarium-produced T-2 toxin promotes tumor growth in mice.
- The dosage and frequency of T-2 toxin administration influence its impact on tumor progression.
- These findings highlight the potential oncogenic effects of T-2 toxin exposure.