Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Whole-body tomographic fluorescence lifetime imaging of PD-L1 expression.

Research square·2026

Same author

Real-Time SLAM-Based Correction and 3D Visualization for Fluorescence Lifetime Imaging.

Medical image computing and computer-assisted intervention : MICCAI ... International Conference on Medical Image Computing and Computer-Assisted Intervention·2026

Same author

High-speed wide-field fluorescence lifetime imaging for intraoperative tumor visualization and in vivo multiplexing.

Biomedical optics express·2025

Same author

Early Experience With Indocyanine Green (ICG) Fluorescent Guided Transoral Robotic Surgery With the Da Vinci SP.

Head & neck·2025

Same author

Fluorescence Lifetime Imaging Enables In Vivo Quantification of PD-L1 Expression and Intertumoral Heterogeneity.

Cancer research·2024

Same author

Miniaturized Fab' imaging probe derived from a clinical antibody: Characterization and imaging in CRISPRi-attenuated mammary tumor models.

iScience·2024

Search research articles

Related Experiment Video

Updated: Dec 21, 2025

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Propagation of time-resolved fluorescence in a diffuse medium: complex analytical derivation.

Anand T N Kumar

Journal of the Optical Society of America. A, Optics, Image Science, and Vision

|May 14, 2020

Summary

Complex analysis of fluorescence in diffusive media reveals distinct signal components. This method enables direct measurement of fluorescence lifetimes shorter than optical diffusion timescales, advancing turbid medium characterization.

More Related Videos

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

Related Experiment Videos

Last Updated: Dec 21, 2025

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

Area of Science:

Biomedical Optics
Complex Analysis
Fluorescence Spectroscopy

Background:

Understanding fluorescence dynamics in optically diffusive media is crucial for applications like biomedical imaging.
Existing methods face challenges in resolving fluorescence lifetimes shorter than the medium's diffusion timescale.
Ultrashort laser pulse excitation is a key technique for probing rapid fluorescence phenomena.

Purpose of the Study:

To develop and validate a complex analytical method for evaluating spatiotemporal fluorescence evolution.
To differentiate between diffusive and fluorescence decay terms based on their unique spatiotemporal behaviors.
To enable direct measurement of short fluorescence lifetimes in turbid media.

Main Methods:

Application of complex analytical methods, including Cauchy's integral theorem, to Fourier integrals of fluorescence signals.
Calculation of time-resolved fluorescence signals for various fluorescence lifetimes and source/detector configurations.
Validation of analytical expressions against numerically computed full time-resolved fluorescence signals.

Main Results:

The integrand of the time-resolved diffuse fluorescence exhibits singularities (branch points and simple poles) in the complex-frequency plane.
Complex analysis separates the fluorescence signal into a spatially broadening diffusive term and a time-independent decay term.
Analytical results accurately match numerical computations, confirming the method's validity.

Conclusions:

The distinct spatiotemporal characteristics of the diffusive and fluorescence terms allow for direct lifetime measurements.
This approach overcomes limitations in measuring fluorescence lifetimes shorter than optical diffusion timescales.
The developed complex analytical framework offers a novel tool for characterizing turbid media.