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Characterization of Pasteurella multocida isolates from rabbits in China
Weifeng Zhu1, Zhiyu Fan1, Rulong Qiu1
1Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, 210014, China; National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210014, China.
This study characterizes Pasteurella multocida from Chinese rabbits, identifying three main clonal complexes. Different complexes correlate with specific virulence factors and disease outcomes like pneumonia and septicemia.
Area of Science:
- Veterinary Microbiology
- Molecular Epidemiology
- Bacterial Pathogenesis
Background:
- Pasteurella multocida causes various diseases in animals and humans.
- Previous studies characterized P. multocida in European rabbits, but not in China.
- Understanding rabbit P. multocida populations is crucial for disease control.
Purpose of the Study:
- To characterize the population structure of P. multocida isolates from rabbits in China.
- To determine the genotypes of virulence factors and assess the virulence of these isolates.
- To associate molecular types with virulence factors and clinical presentations.
Main Methods:
- Multilocus sequence typing (MLST) was used to cluster 30 P. multocida isolates.
- Multiplex PCR determined genotypes of capsule, lipopolysaccharides, HgbB, and PfhA.
- The 50% lethal dose (LD50) was determined in a mouse model to assess virulence.
Main Results:
- Three major clonal complexes (CC9, CC74, ST129) were identified.
- CC9 strains (cpsA(F)L3) were linked to rhinitis; CC74 strains (cpsAL6) to pneumonia and specific virulence genes; ST129 strains (cpsAL1) to high virulence and septicemia.
Conclusions:
- Distinct P. multocida populations exist in Chinese rabbits, associated with specific virulence factors and disease manifestations.
- These findings offer insights into P. multocida epidemiology and pathogenesis in rabbits.
- The characterized strains can inform future pathogenesis and vaccine development studies.
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