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Updated: Dec 21, 2025

Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research
Published on: May 12, 2023
Establishment of two induced pluripotent stem cell line from healthy elderly (IPTi005-A and IPTi007-A)
Lin Zhang1, Qiao Ren2, Gang Liu2
1Beijing Institute of Pharmacology and Toxicology; Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures; Beijing, China; School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University; Shenyang, China.
Abstract:
Two healthy elderly donated their peripheral blood mononuclear cells (PBMCs). The induced pluripotent stem cell lines (IPTi005-A and IPTi007-A) were reprogrammed by episomal vector system with Yamanaka factors OCT4, SOX2, C-MYC and KLF4. Both the two iPSC lines can differentiate into three germ layers determined by embryoid bodies (EBs) differentiation. Flow cytometry analysis showed that more than 90% cells expressed NANOG, OCT4 and SSEA4. Besides, the iPSC lines of IPTi005-A and IPTi007-A were confirmed to exhibit a normal karyotype. In the studies of age-related disease, the iPSC lines can be used as controls.
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