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Updated: Dec 21, 2025
09:39
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
Published on: June 9, 2018
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Combining Bioinformatics and Experiments to Identify CREB1 as a Key Regulator in Senescent Granulosa Cells.
Pei-Hsuan Lin1,2, Li-Te Lin1,3,4, Chia-Jung Li1,3
1Department of Obstetrics and Gynecology, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan.
Diagnostics (Basel, Switzerland)
|May 15, 2020
Summary
Ovarian aging, driven by mitochondrial dysfunction in granulosa cells, is linked to CREB1 gene expression. This gene impacts energy metabolism and oxidative stress, affecting female reproductive health.
Area of Science:
- Reproductive Biology
- Cellular Aging
- Mitochondrial Biology
Background:
- Ovarian aging precedes aging in other female organs, impacting fertility.
- Mitochondrial dysfunction in granulosa cells is implicated in ovarian aging.
- Mechanisms of mitochondrial dysfunction in aging granulosa cells are not fully understood.
Purpose of the Study:
- To investigate the role of CREB1 gene expression in ovarian aging.
- To elucidate the molecular mechanisms of mitochondrial dysfunction in aging granulosa cells.
Main Methods:
- Bioinformatics approaches using public resources.
- Analysis of gene expression in ovary tissue.
- Investigation of CREB1 binding to upstream promoters (PRKAA1, PRKAA2).
Main Results:
- Downregulation of bioenergetic-related genes associated with CREB1.
- Altered expression of genes involved in energy metabolism and ROS production.
- CREB1 binding to PRKAA1/PRKAA2 promoters influences granulosa cell biogenesis.
- Increased SIRT1 and PPARGC1A mRNA levels in aging granulosa cells.
Conclusions:
- CREB1 plays a critical role in oxidative stress-induced senescence in granulosa cells.
- CREB1 reduces mitochondrial function, contributing to ovarian aging.
- Findings highlight CREB1 as a potential target for reproductive health interventions.
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