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Crossed-beam pump-probe microscopy.

Jun Jiang, Warren S Warren, Martin C Fischer

|May 15, 2020

Summary

This summary is machine-generated.

We developed crossed-beam pump-probe microscopy (CBPM) for enhanced imaging. This new method improves axial resolution, offering better depth-resolved imaging, especially with low-NA lenses.

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Area of Science:

Optics and Photonics
Microscopy Techniques
Biomedical Imaging

Background:

Pump-probe microscopy is crucial for dynamic process studies.
Achieving high axial resolution with low numerical aperture (NA) lenses remains a challenge.
Depth-resolved imaging with a large field-of-view is desirable in many applications.

Purpose of the Study:

To introduce a novel pump-probe microscopy method using non-collinear excitation.
To enhance axial resolution, particularly when using low-NA imaging lenses.
To provide an alternative for depth-resolved, large field-of-view imaging.

Main Methods:

Developed and implemented crossed-beam pump-probe microscopy (CBPM).
Utilized non-collinear excitation geometry.
Characterized resolution performance with various low-NA imaging lenses.

Main Results:

Demonstrated proof-of-concept for CBPM.
Quantified resolution improvements using different lenses.
Observed significantly enhanced axial resolution with specific low-NA lenses.

Conclusions:

CBPM offers a viable strategy for improving axial resolution in pump-probe microscopy.
The method is particularly beneficial for imaging applications requiring depth penetration with low-NA optics.
CBPM presents a valuable alternative for achieving depth-resolved, large field-of-view imaging.