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Impaired glymphatic system in secondary degeneration areas after ischemic stroke in rats

Luyi Lin1, Xiaozhu Hao1, Chanchan Li1

  • 1Department of Radiology, Huashan Hospital, Fudan University, No. 12 Middle Urumqi Road, Shanghai 200040, China.

Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association
|May 15, 2020
PubMed
Abstract

Insights

This study shows that the brain

Area of Science:

  • Neuroscience
  • Radiology
  • Biomedical Engineering

Background:

  • Secondary degeneration mechanisms post-ischemic stroke remain unclear.
  • Contrast-enhanced MRI using cisterna magna (CM) injection of Gd-DTPA assesses glymphatic system function.
  • This research investigates glymphatic system changes in stroke-induced secondary degeneration areas.

Purpose of the Study:

  • To evaluate glymphatic system function in remote secondary degeneration areas after ischemic stroke using contrast-enhanced MRI.
  • To compare glymphatic function between ipsilateral and contralateral hemispheres in stroke models.

Main Methods:

  • An ischemic stroke model was created using middle cerebral artery occlusion (MCAO) in Sprague-Dawley rats.
  • Rats were divided into control, acute (1 day post-MCAO), and subacute (7 days post-MCAO) phases.
  • Contrast-enhanced MRI with intrathecal Gd-DTPA injection was used to assess signal-to-noise ratio (SNR) changes in the substantia nigra (SN) and ventral thalamic nucleus (VTN).

Main Results:

  • In the subacute phase, significantly higher SNR was observed in the ipsilateral SN and VTN compared to the contralateral side at 5 and 6 hours post-injection.
  • The time-to-peak SNR was longer in the ipsilateral SN compared to the contralateral side in the subacute phase.
  • Glymphatic function, indicated by Gd-DTPA distribution, was impaired in specific brain regions remote from the initial ischemic injury.

Conclusions:

  • Glymphatic system function is impaired in the ipsilateral substantia nigra and ventral thalamic nucleus following ischemic stroke.
  • This impairment in glymphatic transport may contribute to secondary neural degeneration in remote brain regions.
  • Contrast-enhanced MRI is a valuable tool for assessing glymphatic system dysfunction after stroke.
Keywords:
Glymphatic systemIschemic strokeMRISecondary degeneration

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