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Related Experiment Videos

Lectin-dependent cellular cytotoxicity in man.

B Bonavida, A Robins, A Saxon

    Transplantation
    |March 1, 1977
    PubMed
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    Lectin-dependent cellular cytotoxicity (LDCC) involves human leukocytes targeting cells with phytohemagglutinin. This assay identifies cytotoxic cells and reveals two Fc-bearing cell populations mediate this immune response.

    Area of Science:

    • Immunology
    • Cellular Biology

    Background:

    • Human peripheral blood leukocytes exhibit cytotoxicity towards labeled target cells in the presence of phytohemagglutinin.
    • Lectin-dependent cellular cytotoxicity (LDCC) is a rapid assay that reveals potential cytotoxic cells without mitogen-induced activation.
    • LDCC utilizes non-erythrocyte target cells, offering a distinct approach to studying cell-mediated immunity.

    Purpose of the Study:

    • To characterize the effector cells responsible for lectin-dependent cellular cytotoxicity (LDCC).
    • To investigate the role of different leukocyte populations in mediating LDCC.
    • To explore the potential of LDCC as a tool for assessing cell-mediated immunity.

    Main Methods:

    • Cell fractionation techniques including Ficoll-Hypaque separation, polystyrene bead columns, and nylon fiber columns were employed.

    Related Experiment Videos

  • Effector cell populations were enriched or depleted using E rosette sedimentation and EA monolayer adherence assays.
  • Cytotoxicity assays were performed using 51Cr-labeled target cells in the presence of phytohemagglutinin.

    • Main Results:

    • LDCC activity was retained after removing monocytes, granulocytes, and immunoglobulin-bearing leukocytes.
    • Polymorphonuclear leukocytes lacked LDCC activity, while T cell-enriched and non-T cell fractions showed activity.
    • Depletion of Fc-bearing leukocytes abolished LDCC activity, indicating their crucial role.
    • Two populations of Fc-bearing cells, of both thymus and non-thymus origin, were identified as mediators of LDCC.

    Conclusions:

    • LDCC is mediated by two distinct populations of Fc-bearing leukocytes.
    • The assay's ability to use autologous or syngeneic systems with various target cells highlights its feasibility for assessing cell-mediated immunity.
    • LDCC provides a valuable method for evaluating cellular immune responses.