Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
MicroRNAs01:22

MicroRNAs

23.7K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Large-scale Genomic Landscape and Clinical Outcomes of De Novo and Treatment-emergent Neuroendocrine Prostate Cancer.

European urology open science·2026
Same author

Methods to build and maintain effective clinician-patient relationships for adults with disorders of gut-brain interaction (DGBI): a systematic review.

Journal of gastroenterology·2026
Same author

Cold Sensation and Gastric Accommodation in Functional Dyspepsia: An Exploratory Study of Autonomic Function.

Digestion·2026
Same author

Impact of Isolation Techniques on the Content of Small Extracellular Vesicles.

Journal of extracellular vesicles·2026
Same author

DNA Copy Number Profiling in Extracellular Vesicles as Clinical Biomarkers of High-Grade Serous Ovarian Carcinoma.

Journal of extracellular vesicles·2026
Same author

Small Extracellular Vesicles Released From Human Chemically Induced Liver Progenitors Have the Potential to Improve Liver Fibrosis in Mice.

Gastro hep advances·2026

Related Experiment Video

Updated: Dec 21, 2025

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

250

Circulating microRNAs: Next-generation Cancer Detection.

Juntaro Matsuzaki1, Takahiro Ochiya2

  • 1Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.

The Keio Journal of Medicine
|May 15, 2020
PubMed
Summary

This study developed novel cancer detection tools using serum microRNAs (miRNAs). The research created a database of miRNA biomarker candidates for various cancers, aiding early cancer detection.

Keywords:
biomarkercancerextracellular vesiclemiRNAserum

More Related Videos

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening
08:14

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

16.1K
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
08:12

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

5.8K

Related Experiment Videos

Last Updated: Dec 21, 2025

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

250
MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening
08:14

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

16.1K
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
08:12

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

5.8K

Area of Science:

  • Oncology
  • Biomarker Discovery
  • Molecular Diagnostics

Background:

  • Early cancer detection is critical for disease control and preventing malignant progression.
  • MicroRNAs (miRNAs) are increasingly recognized as potential biomarkers for various cancers.
  • Developing novel diagnostic tools for early cancer detection remains a significant challenge.

Purpose of the Study:

  • To develop novel cancer detection tools utilizing serum microRNAs (miRNAs).
  • To establish a comprehensive serum miRNA database for cancer biomarker discovery.
  • To identify potential miRNA biomarker candidates for various cancer types, including rare cancers.

Main Methods:

  • A nationwide project in Japan (2014-2019) focused on serum miRNA analysis for cancer detection.
  • Collected over 10,000 serum samples from patients with malignant diseases, including ovarian cancer, gliomas, and sarcomas, from the National Cancer Center Biobank.
  • Performed comprehensive miRNA microarray analyses on all collected serum samples.

Main Results:

  • Generated a valuable serum miRNA database containing potential biomarker candidates for each cancer type.
  • Provided insights into miRNA expression profiles across a wide range of cancers.
  • The database serves as a foundation for further research into miRNA-based cancer diagnostics.

Conclusions:

  • The national project successfully created a serum miRNA database for cancer biomarker discovery.
  • The identified miRNA candidates hold promise for the early detection of various cancers.
  • Further research is needed to elucidate the functional roles of these circulating miRNAs in cancer biology.