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Updated: Dec 21, 2025

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Published on: June 23, 2022

Validation of SARS-CoV-2 detection across multiple specimen types.

Garrett A Perchetti¹, Arun K Nalla¹, Meei-Li Huang¹

¹Department of Laboratory Medicine, Virology Division, University of Washington, Seattle, WA, United States.

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology

|May 15, 2020

Summary

A modified laboratory test accurately detects SARS-CoV-2 in various sample types, including respiratory secretions and stool. This assay demonstrates consistent sensitivity across different matrices for reliable COVID-19 diagnosis.

Keywords:

BAL CSF Coronavirus SARS-CoV-2 Sensitivity Specimen type

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Area of Science:

Virology
Molecular Diagnostics
Infectious Diseases

Background:

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused a global pandemic with significant mortality.
The virus exhibits broad tropism, with detection reported in diverse biological specimens.

Purpose of the Study:

To assess the accuracy and sensitivity of a modified CDC-based SARS-CoV-2 assay (N1 and N2 targets).
To evaluate the impact of different sample matrices on the assay's analytical sensitivity.

Main Methods:

Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed.
The study compared SARS-CoV-2 detection in nasal swabs (VTM), bronchoalveolar lavage (BAL), sputum, plasma, cerebrospinal fluid (CSF), stool, phosphate-buffered saline (PBS), and Hanks' Balanced Salt Solution (HBSS).
Limits of detection (LoD) were determined for each specimen type and target gene.

Main Results:

Established LoDs ranged from 10-20 copies/reaction for N1 and 1-10 copies/reaction for N2.
Alternative transport media like PBS and HBSS showed comparable LoDs to viral transport medium (VTM).
The N2 gene target exhibited the highest sensitivity in CSF samples.

Conclusions:

A modified CDC-based assay provides accurate SARS-CoV-2 detection.
The test demonstrates consistent sensitivity across a wide array of tested sample types, supporting its broad clinical utility.