Spectrophotometric method development and validation for simultaneous estimation of Anagliptin and Metformin HCl BY Q...

A simple, accurate, precise and economical Q- Absorption Ratio spectrophotometric method was developed and validated for estimation of Anagliptin and Metformin HCl in synthetic mixture. Anagliptin and Metformin HCl showed an iso-absorptive point at 238 nm in distilled water. The second wavelength used was 233 nm which is λ_max of Metformin HCl in distilled water. The concentration of the drugs was determined by using ratio of absorbance at iso-absorptive point (λ₁ = 238 nm) and at the λ_max of Metformin HCl (λ₂ = 233 nm). This method is linear for both drugs; in range of 2-12 μg/mL at λ₁ (R² = 0.999) and at λ₂ (R² = 0.9998) for Anagliptin, and in the range of 5-30 μg/mL for Metformin HCl found at λ₁ (R² = 0.9995) and at λ₂ (R² = 0.9997). The % Recovery was 100.42-101.83 % of Anagliptin and 99.94-101.63 % of Metformin HCl by standard addition method. The LOD was found to be 0.201 μg/mL and 0.262 μg/mL for Anagliptin at λ₁ and λ₂ respectively. The LOD was found to be 0.320 μg/mL and 0.167 μg/mL for Metformin HCl at λ₁ and λ₂ respectively. The LOQ was found to be 0.610 μg/mL and 0.794 μg/mL for Anagliptin at λ₁ and λ₂ respectively. The LOQ was found to be 0.972 μg/mL and 0.506 μg/mL for Metformin HCl at λ₁ and λ₂ respectively. The method was found to be precise as % RSD was less than 2.00 in Repeatability, Interday and Intraday precision for Anagliptin and Metformin HCl. The % assay of analyte drugs in synthetic mixture was found to be 100.601% of Anagliptin and 100.206 % of Metformin HCl which showed good applicability of the developed method.