Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complement System01:27

Complement System

8.8K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
8.8K
Proteomics01:33

Proteomics

9.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.1K
Nephrotic Syndrome II : Assessment and Medical Management01:26

Nephrotic Syndrome II : Assessment and Medical Management

143
IntroductionNephrotic syndrome is a kidney disorder marked by excessive protein loss in the urine, leading to various systemic complications. This condition often results from damage to the glomeruli—the kidney's filtering units—causing proteinuria, low blood protein levels, and fluid retention. Understanding the assessment, diagnosis, and management of nephrotic syndrome is essential for effective treatment and prevention of further kidney damage.AssessmentPatient History: Document...
143
Nephrotic Syndrome I : Introduction01:24

Nephrotic Syndrome I : Introduction

400
Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of...
400

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Absent Cyclin D1 Expression in Myeloid Sarcomas Distinguishes From Malignant Histiocytic Neoplasms: When Morphologic Ambiguity is Deceptive.

The American journal of surgical pathology·2026
Same author

Mutational Profiling Links Biologically Relevant Variants to Multi-Systemic Rosai-Dorfman Disease.

Blood advances·2026
Same author

MPRIP::PDGFRB fusion identified in a male patient with a myeloid/lymphoid neoplasm with eosinophilia.

Annals of hematology·2026
Same author

Case Report: <i>KMT2A</i> amplification in two adult patients with B-cell acute lymphoblastic leukemia.

Frontiers in oncology·2026
Same author

Pulling teeth: access to dental clearance in patients with cancer eligible for bone-modifying agents.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2026
Same author

Systemic Xanthogranuloma or Erdheim-Chester disease: A systematic review re-examining the classification challenge.

British journal of haematology·2026

Related Experiment Video

Updated: Dec 21, 2025

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
09:43

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

3.4K

Proteomic Analysis of Complement Proteins in Membranous Nephropathy.

Aishwarya Ravindran1, Benjamin Madden2, M Cristine Charlesworth2

  • 1Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.

Kidney International Reports
|May 15, 2020
PubMed
Summary

Membranous nephropathy (MN) involves significant complement system activation, particularly involving C3 and C4 pathways. This finding suggests that anticomplement therapies could be a promising treatment for MN.

Keywords:
complementlaser microdissectionmass spectrometrymembranous nephropathy

More Related Videos

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

525
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
09:12

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Published on: January 18, 2019

9.8K

Related Experiment Videos

Last Updated: Dec 21, 2025

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
09:43

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

3.4K
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

525
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
09:12

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Published on: January 18, 2019

9.8K

Area of Science:

  • Nephrology
  • Immunology
  • Proteomics

Background:

  • Membranous nephropathy (MN) is a leading cause of nephrotic syndrome in adults.
  • Phospholipase A2 receptor (PLA2R)-associated MN and exostosin 1/2 (EXT1/EXT2)-associated MN are the most common forms.
  • Previous studies have not comprehensively analyzed the complement profile in these MN subtypes using proteomics.

Purpose of the Study:

  • To investigate the glomerular complement protein profile in PLA2R-associated MN and EXT1/EXT2-associated MN.
  • To compare complement activation in these MN subtypes with control cases.

Main Methods:

  • Laser microdissection and tandem mass spectrometry (MS/MS) were employed.
  • Glomeruli were dissected from patients with PLA2R-associated MN (n=7), EXT1/EXT2-associated MN (n=21), and control biopsies (n=11).
  • Identified and quantified glomerular complement proteins and regulators.

Main Results:

  • High spectral counts for PLA2R and EXT1/EXT2 confirmed their presence in respective MN types.
  • Both MN types exhibited elevated levels of complement proteins C3, C4, C5, C6, C7, C8, and C9.
  • Complement protein C1 was detected at low levels in EXT1/EXT2-associated MN.
  • Regulators of complement activation, including FH, FHR-1, FHR-5, clusterin, and vitronectin, were increased, while FHR-3, FHR-4, and CD59 were decreased.
  • Key alternative pathway components (FB, properdin) were found at low levels.
  • IgG4 and IgG1 were the predominant IgG subclasses.
  • Control cases showed significantly lower spectral counts for C3, C4, and C5 compared to MN cases.

Conclusions:

  • Significant complement activation, involving C3 and C4 pathways, is a hallmark of both PLA2R- and EXT1/EXT2-associated MN.
  • The study identified specific complement proteins and regulators implicated in MN pathogenesis.
  • These findings support the potential efficacy of anticomplement therapies for treating membranous nephropathy.