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Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
Published on: January 31, 2014
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Effective photoacoustic absorption spectrum for collagen-based tissue imaging
Eunwoo Park1, Yong-Jae Lee1, Changho Lee2
1Gwangju Institute of Science and Technology, Advanced Photonics Research Institute, Gwangju, Republic of Korea.
Journal of Biomedical Optics
|May 15, 2020
Summary
This study identifies optimal wavelengths for collagen photoacoustic imaging (PAI) by analyzing its absorption spectrum. This improves PAI sensitivity for tissues like skin and tendons.
Area of Science:
- Biomedical Optics
- Medical Imaging
- Spectroscopy
Background:
- Collagen imaging is crucial for diagnosing and monitoring orthopedic and dermatological conditions.
- Short-wave infrared (SWIR) photoacoustic imaging (PAI) of collagen is challenging due to spectral overlap with water.
- Optimal wavelength selection is needed for sensitive collagen PAI.
Purpose of the Study:
- To derive an effective photoacoustic (PA) absorption spectrum for collagen.
- To identify optimal wavelengths for high-sensitivity PAI of collagen-based tissues.
Main Methods:
- Measured PA signals from collagen samples across the SWIR band.
- Considered laser excitation and water absorption spectrum to derive collagen's PA absorption spectrum.
- Applied a pulsed fiber laser at 1560 nm.
Main Results:
- Identified PA absorption spectral peaks for collagen at 1200, 1550, and 1700 nm.
- Achieved up to a five-fold increase in PA signal compared to commonly used wavelengths.
- Successfully obtained a 3D PA image of a collagen patch.
Conclusions:
- The derived effective PA absorption spectrum aids in selecting optimal wavelengths for collagen PAI.
- Improved PAI sensitivity for collagen-based samples is achievable.
- This approach enhances diagnostic capabilities in fields utilizing collagen imaging.
