Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

GP130 Regulates Macrophage Polarization and Functional Recovery after Spinal Cord Injury in Rats: A Single-Cell RNA Sequencing Study.

Inflammation·2026

Same author

Neuroprotective Effect of Delicaflavone in Rotenone-induced Parkinson's Disease in Rats: Role of Nrf2/HO-1, NF-κB and PI3K/Akt/mTOR Pathways.

Molecular neurobiology·2026

Same author

Nutritional status in maintenance hemodialysis patients in a large Chinese region : a multi-center cross-sectional survey.

Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation·2026

Same author

Runx1-Snx9 axis drives the pathological secretion of mitochondrial-derived vesicles to activate cGAS-STING signaling in acute pancreatitis.

Journal of nanobiotechnology·2026

Same author

Margin-aware prototype learning for client withdrawal in federated unlearning.

Neural networks : the official journal of the International Neural Network Society·2026

Same author

Evaluating Pay-It-Forward Strategy to Promote Hepatitis B Virus and Hepatitis C Virus Testing Among International Migrants From Low- and Middle-Income Countries in China: Protocol for a Cluster Randomized Controlled Trial.

JMIR research protocols·2026

Search research articles

Related Experiment Video

Updated: Dec 21, 2025

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell-Drug Interactions.

Shaofei Shen¹, Fangjuan Zhang¹, Mengqi Gao¹

¹College of Life Science, Shanxi Agricultural University, Taigu 030801, China.

|May 16, 2020

Summary

This study introduces a novel microfluidic device for efficient drug screening in cancer therapy. The device rapidly creates wide-ranging drug concentration gradients, enabling accurate real-time analysis of tumor cell-drug interactions.

Keywords:

concentration gradient drug screening inertial microfluidics microfluidic chip spiral mixer

More Related Videos

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Published on: September 19, 2019

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Published on: May 30, 2025

Related Experiment Videos

Last Updated: Dec 21, 2025

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Published on: September 19, 2019

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Published on: May 30, 2025

Area of Science:

Biomedical Engineering
Cancer Research
Microfluidics

Background:

Conventional cancer models face ethical, time, and cost limitations.
Microfluidic devices offer advantages for drug screening, but often have limited concentration ranges and flow rates.
Developing simple, wide-range concentration gradient devices for real-time tumor cell-drug interaction studies is challenging.

Purpose of the Study:

To develop a simple, compact microfluidic device for constructing efficient and reliable drug-concentration gradients over a wide range of flow rates.
To systematically and quantitatively investigate the dynamic formation of concentration gradients using spiral mixer regulations.
To evaluate the efficacy of anticancer drugs against multiple tumor cell lines simultaneously using the developed device.

Main Methods:

A novel, simple, and compact microfluidic device was designed and fabricated.
Successive spiral mixer regulations were employed to dynamically form concentration gradients.
The device was used to generate accurate, stable, and controllable dual drug-concentration gradients.
The efficacy of paclitaxel was evaluated against human breast adenocarcinoma and human cervical carcinoma cell lines.

Main Results:

The microfluidic device successfully constructed efficient and reliable drug-concentration gradients across a wide flow rate range.
Dual drug-concentration gradients were accurately, stably, and controllably produced.
Paclitaxel demonstrated dose-dependent effects on both human breast adenocarcinoma and human cervical carcinoma cell lines under identical conditions.

Conclusions:

The developed microfluidic device offers a simple and effective method for generating wide-ranging drug-concentration gradients.
This technology facilitates real-time, high-throughput, and accurate analysis of tumor cell-drug interactions.
The device has the potential to advance microfluidic chip development for portable and economical biochemical research in cancer therapy.