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Updated: Dec 21, 2025

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

Cell-modified bioprinted microspheres for vascular regeneration.

Jian Shen¹, Yongli Ji¹, Mingjun Xie²

¹Department of Cardiology, Cardiovascular Key Lab of Zhejiang Province, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310000, China.

Materials Science & Engineering. C, Materials for Biological Applications

|May 16, 2020

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This study developed electrospray bioprinting for cell therapy, creating hydrogel microspheres that promote blood vessel growth in limb ischemia models with good safety. Bioprinting shows promise for future cell therapies.

Area of Science:

Biotechnology
Regenerative Medicine
Vascular Biology

Background:

Cell therapy offers therapeutic potential but faces challenges like immunogenicity and efficacy.
Therapeutic angiogenesis aims to restore blood flow in conditions like limb ischemia.
Existing cell and gene therapies have limitations hindering clinical translation.

Purpose of the Study:

To develop a novel electrospray bioprinting method for creating cell-laden hydrogel microspheres.
To evaluate the safety and efficacy of these microspheres for promoting therapeutic angiogenesis.
To assess the potential of bioprinted cell therapies for treating limb ischemia.

Main Methods:

Electrospray bioprinting was used to generate hydrogel microspheres encapsulating VEGF-overexpressing HEK293T cells.

Keywords:

3D bioprinting Cell therapy Therapeutic angiogenesis VEGF control release

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Last Updated: Dec 21, 2025

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Published on: May 19, 2022

The safety of subcutaneous injection of these microspheres was assessed in C57BL/6 mice.

Angiogenesis was evaluated in a mouse hind-limb ischemia model.

Main Results:

The developed method rapidly produced cell-laden hydrogel microspheres.
Subcutaneous injection of microspheres demonstrated good safety in mice.
The cell-modified microspheres effectively promoted angiogenesis in the limb ischemia model.

Conclusions:

Electrospray bioprinting is a viable method for creating cell-laden microspheres for therapeutic applications.
This approach shows significant potential for inducing angiogenesis and treating limb ischemia.
Bioprinting holds a promising future in the field of cell therapy.