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Modification of chlorosulfonated polystyrene substrates for bioanalytical applications.
Beatriz Díez-Buitrago1, F J Fernández-SanArgimiro2, Jaione Lorenzo2
1CIC biomaGUNE, Paseo Miramón 182, 20014 Donostia-San Sebastián, Spain; Tecnalia Research & Innovation, Paseo Mikeletegi 2, 20009 Donostia-San Sebastián, Spain.
Summary
This study details modifying polystyrene (PS) micro-well plates for bioanalysis. Aminated PS surfaces demonstrated superior performance in immunoassays compared to other modifications and commercial kits.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Analytical Chemistry
Background:
- Polystyrene (PS) micro-well plates are widely used in bioanalytical applications.
- Surface modification is crucial for enhancing the performance of bioanalytical platforms.
- Developing cost-effective and efficient surface modification strategies is essential.
Purpose of the Study:
- To describe the modification of polystyrene micro-well plates using a wet-chemical procedure.
- To evaluate the modified plates as a bioanalytical platform for immunoassays.
- To compare the performance of different surface functionalizations and attachment strategies.
Main Methods:
- Chlorosulfonation of polystyrene substrates to create reactive surfaces.
- Conversion of chlorosulfonyl groups into various functional groups (e.g., amine, carboxyl, alkene, epoxy).
- Characterization using contact angle measurements, X-ray Photoelectron Spectroscopy, and colorimetry.
- Immobilization of antibodies via covalent linkage and direct adsorption.
- Performance evaluation using immunoassays, including comparison with a commercial Human Serum Albumin ELISA kit.
Main Results:
- The wet-chemical chlorosulfonation method produced transparent, stable, and well-controlled reactive polystyrene surfaces under ambient conditions.
- Surface modification increased hydrophilicity, improving interaction with biocompounds.
- Aminated surfaces exhibited superior performance in immunoassays compared to carboxyl, alkene, or epoxy functionalized surfaces.
- The modified PS platform, particularly with aminated surfaces, outperformed a commercial Human Serum Albumin ELISA kit.
Conclusions:
- The described wet-chemical modification offers a versatile and effective method for preparing advanced bioanalytical platforms from polystyrene micro-well plates.
- Aminated polystyrene surfaces provide an optimal platform for immunoassays, demonstrating enhanced sensitivity and performance.
- This approach presents a promising alternative to existing commercial kits for various bioanalytical applications.
