Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Six Drivers of Aging Identified Among Genes Differentially Expressed With Age.

Aging cell·2025

Same author

Benefits of early intervention with olipudase alfa in symptomatic children with acid sphingomyelinase deficiency: A sibling case-comparison study.

Molecular genetics and metabolism reports·2025

Same author

Similarities and differences in the gene expression signatures of physiological age versus future lifespan.

Aging cell·2024

Same author

Six drivers of aging identified among genes differentially expressed with age.

bioRxiv : the preprint server for biology·2024

Same author

Proximity proteomic analysis of the NRF family reveals the Parkinson's disease protein ZNF746/PARIS as a co-complexed repressor of NRF2.

Science signaling·2023

Same author

Functional trajectories during innate spinal cord repair.

Frontiers in molecular neuroscience·2023

Search research articles

Related Experiment Video

Updated: Dec 21, 2025

Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions

Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions

Published on: December 2, 2022

A Simple Apparatus for Individual C. elegans Culture.

William E Pittman^1,2, Drew B Sinha^1,2, Holly E Kinser^1,2

¹Department of Developmental Biology and Department of Genetics, Washington University in St. Louis, St. Louis, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 16, 2020

Summary

Researchers developed a "worm corral" to individually culture Caenorhabditis elegans (C. elegans) throughout their lifespan. This accessible system enables high-resolution microscopy for studying C. elegans development and phenotypes.

Keywords:

C. elegans Culture system Lifespan Longitudinal imaging

More Related Videos

Long-Term Culture and Monitoring of Isolated Caenorhabditis elegans on Solid Media in Multi-Well Devices

Long-Term Culture and Monitoring of Isolated Caenorhabditis elegans on Solid Media in Multi-Well Devices

Published on: December 9, 2022

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

Published on: April 11, 2022

Related Experiment Videos

Last Updated: Dec 21, 2025

Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions

Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions

Published on: December 2, 2022

Long-Term Culture and Monitoring of Isolated Caenorhabditis elegans on Solid Media in Multi-Well Devices

Long-Term Culture and Monitoring of Isolated Caenorhabditis elegans on Solid Media in Multi-Well Devices

Published on: December 9, 2022

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

Published on: April 11, 2022

Area of Science:

Developmental Biology
Genetics
Microscopy

Background:

Standard Caenorhabditis elegans (C. elegans) culture methods often house worms in groups, limiting individual observation.
Observing individual C. elegans throughout their entire life cycle is crucial for detailed phenotypic and developmental studies.

Purpose of the Study:

To develop a miniaturized, accessible system for long-term, individual culture of C. elegans.
To enable high-resolution imaging of individual C. elegans from hatching to death.

Main Methods:

Miniaturization of solid-phase culture conditions for C. elegans.
Development of the "worm corral" device for housing individual nematodes.
Compatibility testing with high-resolution brightfield and fluorescent microscopy.

Main Results:

The "worm corral" system successfully houses individual C. elegans throughout their lifespan.
The system allows for high-resolution imaging of fluorescent transgenes and morphological phenotypes.
The culture devices are easily constructed using standard laboratory equipment and reagents.

Conclusions:

The "worm corral" provides an attainable solution for individual C. elegans culture and live imaging.
This method facilitates detailed studies of C. elegans development, genetics, and phenotypes.
The system's accessibility makes it suitable for most research laboratories.