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Updated: Dec 21, 2025
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Detection of HNE Modification of Proteins in Aging Mouse Tissues: A Western Blot-Based Approach
Hongqiao Zhang1, Natalie Lyn2, Amin Haghani2
1Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA. hongqiaz@usc.edu.
Abstract:
4-Hydroxenonenal (HNE) is one of the major α,β-unsaturated aldehyde products of lipid peroxidation. HNE can form conjugates with macromolecules, including protein, and thereby alter their function. HNE and its conjugation with proteins are increased in aging and age-related diseases. To elucidate how HNE is involved in these aging-related pathophysiological changes, it is necessary to assess HNE modification of proteins. Here a simple and convenient Western-blot based method is presented to detect HNE modification of proteins in tissues of aging mice.
