Subclinical keratoconus detection with three-dimensional (3-D) morphogeometric and volumetric analysis

To assess the efficacy of morphogeometric and volumetric characterization of the cornea based on three-dimensional (3-D) modelling in diagnosis of subclinical keratoconus (KC).

Cross-sectional study. Ninety-three eyes with subclinical KC with a best spectacle-corrected distance visual acuity ≥20/20 (grade zero KC according to the RETICS classification) and 109 control eyes were included. Computer-based 3-D corneal morphogeometric model was generated using raw topographic data. Distance-, area- and volume-based parameters were used for statistical analysis. Distance parameters included deviation of anterior (D_apexant )/posterior (D_apexpost ) apices and minimum thickness points (D_mctant , D_mctpost ) from corneal vertex, and D_apexant -D_apexpost difference. Areal variables were derived from anterior (A_ant ) and posterior (A_post ) corneal surfaces, sagittal plane passing through corneal apices (A_apexant , A_apexpost ) and thinnest point (A_mctant , A_mctpost ). Total corneal volume (V_total ) and volumetric distribution (with 0.1mm steps) centred to thinnest corneal point (VOL_mct ) and anterior (VOL_aap )/posterior (VOL_pap ) apices comprised the volume-based parameters.

In the subclinical KC group, all D values, D_apexant -D_apexpost difference, A_ant , A_post and A_apexant values were higher (p < 0.001), while A_apexpost , A_mctpost , V_total , VOL_mct , VOL_aap and VOL_pap values were lower when compared to the control group (p < 0.001). Regression analysis-based formula correctly classified 96.8% of the eyes with subclinical KC and 94.5% of the normal ones (p < 0.0001).

Eyes with subclinical KC seem to represent asymmetrically displaced anterior and posterior corneal apex, corneal thinning and volume loss. 3-D morphogeometric and volumetric parameters and differentiation formula can be incorporated into topography software to detect subclinical KC with high sensitivity and specificity in clinical practice.