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Long non-coding RNA OIP5-AS1 suppresses multiple myeloma progression by sponging miR-27a-3p to activate TSC1

Yong Wang1, Haibao Wang1, Jianwei Ruan1

  • 1Department of Orthopaedic, Taizhou Municipal Hospital, No. 381, Zhongshan East Road, Jiaojiang District, Taizhou, 318000 Zhejiang China.

Cancer Cell International
|May 16, 2020
PubMed
Abstract

Insights

Long noncoding RNA OIP5-AS1 inhibits multiple myeloma progression by regulating the miR-27a-3p/TSC1 pathway. This finding offers potential therapeutic targets for treating this hematological malignancy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Multiple myeloma (MM) is a common blood cancer.
  • Long noncoding RNAs (lncRNAs) play a role in MM development.
  • The specific function of lncRNA OIP5-AS1 in MM was investigated.

Purpose of the Study:

  • To explore the function of OIP5-AS1 in multiple myeloma.
  • To elucidate the underlying molecular mechanism involving miR-27a-3p and TSC1.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • Cell proliferation, apoptosis, and metastasis assays (CCK-8, colony formation, BrdU, flow cytometry, transwell).
  • Dual-luciferase reporter and RNA immunoprecipitation assays to confirm molecular interactions; in vivo tumor xenograft assay.

Main Results:

  • OIP5-AS1 and TSC1 expression were decreased, while miR-27a-3p was upregulated in MM.
  • OIP5-AS1 overexpression inhibited MM cell viability, proliferation, migration, invasion, and tumorigenesis, while promoting apoptosis.
  • OIP5-AS1 acts as a sponge for miR-27a-3p, upregulating TSC1 and thereby inhibiting MM progression.

Conclusions:

  • OIP5-AS1 suppresses multiple myeloma progression.
  • The OIP5-AS1/miR-27a-3p/TSC1 axis is a key regulatory pathway in MM.
  • This pathway represents a potential therapeutic target for multiple myeloma treatment.
Keywords:
Multiple myelomaTSC1lncRNA OIP5-AS1miR-27a-3p

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